EMPIAR-12524 - Structure of the 55LCC ATPase complex [multiple data sets in EER and MRC formats]

EMPIAR-12524

Structure of the 55LCC ATPase complex [multiple data sets in EER and MRC formats]

Publication:

The SPATA5-SPATA5L1 ATPase complex directs replisome proteostasis to ensure genome integrity

Krishnamoorthy V, Foglizzo M, Dilley RL, Wu A, Datta A, Dutta P, Campbell LJ, Degtjarik O, Musgrove LJ, Calabrese AN, Zeqiraj E, Greenberg RA

Cell 9 (2024) 2250-2268

PMID: 38554706

DOI: 10.1016/j.cell.2024.03.002

Related EMDB entry:

EMD-19177

Deposited:

2025-01-11

Released:

2025-06-27

Last modified:

2025-06-27

Imageset size:

9.66 TB

Imageset DOI:

10.6019/EMPIAR-12524

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
micrographs - single frame

Image Sets

1. Unaligned multiframe micrographs in the EER format of the 55LCC ATPase complex

Category:

micrographs - multiframe

Image format:

EER

No. of images or tilt series:

27595

Image size:

(None, None)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(0.74, 0.74)

Details:

A dose per physical pixel/s of 6.88 was used, resulting in a total electron dose of 37.6 e-/Å2, fractionated across 918 EPU frames. These were then grouped into 20 frames, resulting in an electron dose of 0.8 e-/Å2 per frame.

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2. Aligned micrographs of of the 55LCC ATPase complex

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

27595

Image size:

(None, None)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.74, 0.74)

Details:

Raw movie frames are aligned with RELION's own implementation of the MotionCor2 algorithm using an EER fractionation of 47.

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

See all citations

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