EMPIAR-12493 - Cryo-EM structures of TUG-1375/4-CMTB-bound active FFA2 and GLPG0974-bound inactive FFA2 [multiple data sets in TIFF format]

EMPIAR-12493

Cryo-EM structures of TUG-1375/4-CMTB-bound active FFA2 and GLPG0974-bound inactive FFA2 [multiple data sets in TIFF format]

Publication:

Structural insights into lipid chain-length selectivity and allosteric regulation of FFA2

Kise R, Kojima A, Inoue WJ, Matsui TE, Fukunaga A, Kobayashi J, Kim S, Ikeda H, Yamashita K, Saito K, Ishikita H, Dror RO, Inoue A, Kato HE, Kawakami K, Suomivuori CM, Tsujimura M, Kobayashi K, Kojima A, Fukuda M, Matsui TE, Fukunaga A, Koyanagi J, Kim S, Ikeda H, Saito K, Inoue A

Nature communications 16 (2025)

PMID: 40140663

DOI: 10.1038/s41467-025-57983-4

Related EMDB entry:

EMD-39003 EMD-39004

Deposited:

2024-12-05

Released:

2025-06-27

Last modified:

2025-06-27

Imageset size:

5.67 TB

Imageset DOI:

10.6019/EMPIAR-12493

Experimental metadata:

Download xml json

Deposited via PDBj

Contains:

micrographs - multiframe

Image Sets

1. TUG-1375 and 4-CMTB-bound human FFA2 in complex with Gi

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

8758

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.83, 0.83)

Details:

- Please use "gain_reference/movie_gain.mrc" as gain reference file.
- Please ignore the pixel size in the header (1.00 Å/px); use 0.83 Å/px.
- Raw pixel size: 0.83 A
- Accelerating voltage: 300 kV
- Spherical aberration: 2.7 mm
- Total exposure dose: 45.579 e/A^2

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2. GLPG0974-bound human FFA2

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

7474

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.83, 0.83)

Details:

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

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3. GLPG0974-bound human FFA2

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

10021

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.83, 0.83)

Details:

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

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Citations

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Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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