EMPIAR-12493
Cryo-EM structures of TUG-1375/4-CMTB-bound active FFA2 and GLPG0974-bound inactive FFA2 [multiple data sets in TIFF format]
Publication:

Structural insights into lipid chain-length selectivity and allosteric regulation of FFA2

Kise R, Kojima A, Inoue WJ, Matsui TE, Fukunaga A, Kobayashi J, Kim S, Ikeda H, Yamashita K, Saito K, Ishikita H, Dror RO, Inoue A, Kato HE, Kawakami K, Suomivuori CM, Tsujimura M, Kobayashi K, Kojima A, Fukuda M, Matsui TE, Fukunaga A, Koyanagi J, Kim S, Ikeda H, Saito K, Inoue A

Nature communications 16 (2025)

PMID: 40140663

Related EMDB entry:
Deposited:
2024-12-05
Released:
2025-06-27
Last modified:
2025-06-27
Imageset size:
5.67 TB
Imageset DOI:
Experimental metadata:
Download xml json
Deposited via PDBj
Contains:
  • micrographs - multiframe
1. TUG-1375 and 4-CMTB-bound human FFA2 in complex with Gi
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
8758
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.83, 0.83)
Details:
- Please use "gain_reference/movie_gain.mrc" as gain reference file.
- Please ignore the pixel size in the header (1.00 Å/px); use 0.83 Å/px.
- Raw pixel size: 0.83 A
- Accelerating voltage: 300 kV
- Spherical aberration: 2.7 mm
- Total exposure dose: 45.579 e/A^2
Files:
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2. GLPG0974-bound human FFA2
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
7474
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.83, 0.83)
Details:
- Please use "gain_reference/movie_gain.mrc" as gain reference file.
- Please ignore the pixel size in the header (1.00 Å/px); use 0.83 Å/px.
- Raw pixel size: 0.83 A
- Accelerating voltage: 300 kV
- Spherical aberration: 2.7 mm
- Total exposure dose: 45.589 e/A^2
Files:
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3. GLPG0974-bound human FFA2
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
10021
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.83, 0.83)
Details:
- Please use "gain_reference/movie_gain.mrc" as gain reference file.
- Please ignore the pixel size in the header (1.00 Å/px); use 0.83 Å/px.
- Raw pixel size: 0.83 A
- Accelerating voltage: 300 kV
- Spherical aberration: 2.7 mm
- Total exposure dose: 57.9 e/A^2
Files:
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Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)
Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)
Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)
Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)
Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)