EMPIAR-12332 - Cryo-EM structure of the barley Yellow stripe 1 transporter [multiple data sets in TIFF format]

EMPIAR-12332

Cryo-EM structure of the barley Yellow stripe 1 transporter [multiple data sets in TIFF format]

Publication:

Uptake mechanism of iron-phytosiderophore from the soil based on the structure of yellow stripe transporter

Yamagata A, Murata Y, Namba K, Terada T, Fukai S, Shirouzu M

Nature communications 13 (2022)

PMID: 36424382

DOI: 10.1038/s41467-022-34930-1

Related EMDB entry:

EMD-32767 EMD-32765 EMD-32766

Deposited:

2024-09-18

Released:

2025-07-06

Last modified:

2025-07-06

Imageset size:

5.04 TB

Imageset DOI:

10.6019/EMPIAR-12332

Experimental metadata:

Download xml json

Deposited via PDBj

Contains:

micrographs - multiframe

Image Sets

1. [EMD-32765 / 7WSR] Cryo-EM structure of the barley Yellow stripe 1 transporter

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

5278

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.8285, 0.8285)

Details:

- Microscope: FEI TITAN Krios
- Detector: Gatan K3 BIOQUANTUM
- Acceleration voltage (kV): 300
- The pixel size in the image header (1 Å/px) is wrong. 0.8285 Å/px is the calibrated value.
- Spherical aberration (Cs) : 2.7
- Nominal Defocus (μm): 0.8 - 2.5
- Average electron dose per image (e-/A2): 50.3
- Number of frames per image: 50
- Please use 'gain.mrc' as a gain reference without rotation and y-flipping.

Files:

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2. [EMD-32766 / 7WST] Cryo-EM structure of the barley Yellow stripe 1 transporter in complex with Fe(III)-DMA

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

11930

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.8285, 0.8285)

Details:

- Microscope: FEI TITAN Krios
- Detector: Gatan K3 BIOQUANTUM
- Acceleration voltage (kV): 300
- The pixel size in the image header (1 Å/px) is wrong. 0.8285 Å/px is the calibrated value.
- Spherical aberration (Cs) : 2.7
- Nominal Defocus (μm): 0.6 - 2.5
- Average electron dose per image (e-/A2): 51.4
- Number of frames per image: 48
- Please use 'gain.mrc' as a gain reference without rotation and y-flipping.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. [EMD-32767 / 7WSU] Cryo-EM structure of the barley Yellow stripe 1 transporter in complex with Fe(III)-PDMA

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

6027

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.8285, 0.8285)

Details:

- Microscope: FEI TITAN Krios
- Detector: Gatan K3 BIOQUANTUM
- Acceleration voltage (kV): 300
- The pixel size in the image header (1 Å/px) is wrong. 0.8285 Å/px is the calibrated value.
- Spherical aberration (Cs) : 2.7
- Nominal Defocus (μm): 0.8 - 2.5
- Average electron dose per image (e-/A2): 49.8
- Number of frames per image: 48
- Please use 'gain.mrc' as a gain reference without rotation and y-flipping.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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