EMPIAR-12308 - Electron Cryomicroscopy of alpha-Latrotoxin [87895 micrographs in MRC format]

EMPIAR-12308

Electron Cryomicroscopy of alpha-Latrotoxin [87895 micrographs in MRC format]

Publication:

Molecular mechanism of α-latrotoxin action

Klink B, Alavizargar A, Subramaniam K, Chen M, Heuer A, Gatsogiannis C

bioRxiv (2024)

DOI: 10.1101/2024.03.06.583760

Related EMDB entry:

EMD-51494 EMD-51495 EMD-51490 EMD-51475 EMD-51488 EMD-51484 EMD-51492 EMD-51485 EMD-51479 EMD-51474

Deposited:

2024-08-16

Released:

2025-07-10

Last modified:

2025-07-10

Imageset size:

2.68 TB

Imageset DOI:

10.6019/EMPIAR-12308

Experimental metadata:

Download xml json

Contains:

micrographs - single frame

Image Sets

1. Motion corrected micrographs of alpha-LTX

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

87895

Image size:

(4096, 4096)

Pixel type:

16 BIT FLOAT

Pixel spacing:

(0.58, 0.58)

Details:

Particle positions in corresponding .box text files (with X and Y coordinates in 1st and second column respectively). The micrographs are the motion corrected average of 49-51 movie fractions, which were themself created from 918-1225 EER movie frames. The accumulated electron dose was ~50 electrons per square Angstroem. The coordinates for the aLTX Pore and Prepore state are located in the respective folders data/aLTX_Coordinates_Pore_4_EMPIAR and data/aLTX_Coordinates_Prepore_4_EMPIAR.

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Citations

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Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

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