EMPIAR-12287 - CryoEM ice images and labels used for VirtualIce [multiple data sets in MRC, MRCS and JPEG formats]

EMPIAR-12287

CryoEM ice images and labels used for VirtualIce [multiple data sets in MRC, MRCS and JPEG formats]

Publication:

VirtualIce: Half-synthetic CryoEM Micrograph Generator

Noble AJ

bioRxiv (2024)

DOI: 10.1101/2024.09.28.615520

Related EMDB entry:

Deposited:

2024-09-05

Released:

2024-10-11

Last modified:

2024-10-11

Imageset size:

308.55 GB

Imageset DOI:

10.6019/EMPIAR-12287

Experimental metadata:

Download xml json

Contains:

micrographs - single frame
micrographs - multiframe

Image Sets

1. Micrographs of vitrified buffer with labels of objects that can obscure particles

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

678

Image size:

(3838, 3710)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.096, 1.096)

Details:

Frame-aligned micrographs of vitrified buffer with associated labels (.json files in AnyLabeling v0.3.3 format) of objects that can obscure particles.
This directory must either go in your VirtualIce installation directory for it to work automatically or in a different directory and pointed to with VirtualIce.

Files:

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2. Frame stacks of vitrified buffer

Category:

micrographs - multiframe

Image format:

MRCS

No. of images or tilt series:

900

Image size:

(3838, 3710)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(1.096, 1.096)

Details:

Bzip2 compressed frame stacks of vitrified buffer. Collected on a Krios, Cs corrector, BioQuantum-K2 (15eV slit width), 50.81 e-/Å2 total dose, nominal defocus range randomly chosen between 1 and 3 µm. References included.

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3. Micrographs of vitrified buffer

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

897

Image size:

(3838, 3710)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.096, 1.096)

Details:

Frame-aligned micrographs of vitrified buffer.

Files:

This entry cannot be downloaded from PDBj.

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4. Downsampled micrographs of vitrified buffer tinted green with labels of objects that can obscure particles

Category:

micrographs - single frame

Image format:

JPEG

No. of images or tilt series:

900

Image size:

(960, 928)

Pixel type:

16 BIT FLOAT

Pixel spacing:

(4.384, 4.384)

Details:

Frame-aligned, downsampled by 4 micrographs of vitrified buffer converted to JPEG and tinted green to expedite the visualization process. Associated labels (.json files in AnyLabeling v0.3.3 format) of objects that can obscure particles are included.

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Browse All Files

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Citations

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Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

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Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

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