EMPIAR-12217 - Wild type ATTR amyloid fibril from senile systemic amyloidosis [1943 multi-frame micrographs composed of 40 frames each in TIFF format]

EMPIAR-12217

Wild type ATTR amyloid fibril from senile systemic amyloidosis [1943 multi-frame micrographs composed of 40 frames each in TIFF format]

Publication:

Cryo-EM structure of an ATTRwt amyloid fibril from systemic non-hereditary transthyretin amyloidosis

Steinebrei M, Gottwald J, Baur J, Röcken C, Hegenbart U, Schönland S, Schmidt M

Nature communications 13 (2022)

PMID: 36302762

DOI: 10.1038/s41467-022-33591-4

Related EMDB entry:

EMD-15361

Deposited:

2024-08-06

Released:

2025-02-04

Last modified:

2025-02-04

Imageset size:

332.20 GB

Imageset DOI:

10.6019/EMPIAR-12217

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multiframe micrographs of ATTRwt amyloid fibrils

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

1943

Image size:

(3838, 3710)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(1.04, 1.04)

Details:

Krios1 Quantum-K2
Cs 2.7
300kV EFTEM nanpprobe
x130k spot 8 beam size 620nm
c2=50 objective_aperture=100

GIF-K2 counting
12s exposure 40 frames
total dose at the sample 44.90e/A2
1 pixel = 1.04A
dose rate ~3 e/pix/s
defocus range -1.2 to -2.5

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

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Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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