EMPIAR-11896 - Cryo-electron tomography data of Emiliania huxleyi virus 201 for subtomogram averaging [283 tilt series in TIFF format]

EMPIAR-11896

Cryo-electron tomography data of Emiliania huxleyi virus 201 for subtomogram averaging [283 tilt series in TIFF format]

Publication:

Structure and replication cycle of a virus infecting climate-modulating alga Emiliania huxleyi

Homola M, Büttner CR, Füzik T, Nováček J, Chaillet M, Förster F, Plevka P

Science Advances (2024)

DOI: 10.1101/2023.06.30.547180

Related EMDB entry:

EMD-17649

Deposited:

2024-02-08

Released:

2024-04-12

Last modified:

2024-04-12

Imageset size:

294.76 GB

Imageset DOI:

10.6019/EMPIAR-11896

Experimental metadata:

Download xml json

Contains:

tilt series

Image Sets

1. Raw multiframes movies of EhV-201 particles tilt-series

Category:

tilt series

Image format:

TIFF

No. of images or tilt series:

283

Image size:

(5760, 4092)

Pixel type:

BIT

Pixel spacing:

(2.08, 2.08)

Details:

Dataset contains 283 tilt-series, each consisting of 33 tilts (+/- 48 deg with 3 deg increment), each containing 6 frames. Dataset details: Titan Krios G2 (Thermofisher Scientific) 300 kV; detector K3 with Bioquantum energy filter (Gatan); Pixel size: 2.08; Total dose: 80 e/A^2; Dose-symmetric collection scheme. Data are not motion-corrected nor gain-corrected. Each tilt "fileName.tif" is accompanied by "fileName.tif.mdoc" file with acquisition details.
Gain file: data/Gain
mdoc tomo files: data/mdoc (to assemble the tilt-series from the individual tilts)
More information about the sample preparation and data acquisition can be found in the article (Homola et al. 2024 - Structure and replication cycle of a virus infecting climate-modulating alga Emiliania huxleyi) or in the EMDB deposition 17649.

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