EMPIAR-11837 - Cryo-EM of MERS 5' proximal stem-loop 5 [multiple data sets in MRC format]

EMPIAR-11837

Cryo-EM of MERS 5' proximal stem-loop 5 [multiple data sets in MRC format]

Publication:

Tertiary folds of the SL5 RNA from the 5' proximal region of SARS-CoV-2 and related coronaviruses

Kretsch RC, Xu L, Zheludev IN, Zhou X, Huang R, Nye G, Li S, Zhang K, Chiu W, Das R

Proceedings of the National Academy of Sciences of the United States of America 121 (2024)

PMID: 38427602

DOI: 10.1073/pnas.2320493121

Related EMDB entry:

EMD-42810 EMD-42811 EMD-42809

Deposited:

2023-11-28

Released:

2024-04-17

Last modified:

2024-04-17

Imageset size:

9.92 TB

Imageset DOI:

10.6019/EMPIAR-11837

Experimental metadata:

Download xml json

Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

MRC movies (exposures/*/FoilHole_*_Fractions.mrc) in this entry were compressed by bzip2 (pbzip2)
by EMPIAR Japan to save space and bandwidth.

Some programs (e.g. RELION >= 4.0.1 and CryoSPARC) can process compressed movies without explicit
decompression. For other programs, movies have to be decompressed beforehand. In this case,
you might want to convert decompressed MRC movies into TIFF to save your local storage.

Original size:   17,359,586,244 kilobytes
Compressed size: 10,041,980,776 kilobytes

Contains:

micrographs - multiframe
picked particles - single frame - processed

Image Sets

1. Unaligned multi-frame micrographs of MERS 5' stem-loops 5 RNA.

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

13244

Image size:

(4096, 4096)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(0.82, 0.82)

Details:

These micrographs were collected with a Titan Krios G3i, 300kV and a Falcon 4 detector. Over the collection, the sample was exposed evenly to 49.97 electrons per angstrom squared with a total exposure time of 4.74 seconds. Desired defocuses ranged from -1 to -2 nm. The exposures are gain corrected.

Files:

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2. Extracted particles and orientations for final reconstruction of MERS 5' stem-loop 5 RNA.

Category:

picked particles - single frame - processed

Image format:

MRC

No. of images or tilt series:

286094

Image size:

(360, 360)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.82, 0.82)

Details:

Particles extracted from motion-corrected micrographs. The orientations for the final reconstruction can be found in data/MERS/particles/conformation*.cs for all 3 conformations.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

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Citations

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Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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