EMPIAR-11835 - Cryo-EM of HCoV-229E 5' proximal stem-loop 5 [multiple data sets in TIFF and MRC formats]

EMPIAR-11835

Cryo-EM of HCoV-229E 5' proximal stem-loop 5 [multiple data sets in TIFF and MRC formats]

Publication:

Tertiary folds of the SL5 RNA from the 5′ proximal region of SARS-CoV-2 and related coronaviruses

Kretsch RC, Xu L, Zheludev IN, Zhou X, Huang R, Nye G, Li S, Zhang K, Chiu W, Das R

bioRxiv (2023)

DOI: 10.1101/2023.11.22.567964

Related EMDB entry:

EMD-42803

Deposited:

2023-11-28

Released:

2025-07-10

Last modified:

2025-07-10

Imageset size:

19.60 TB

Imageset DOI:

10.6019/EMPIAR-11835

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
picked particles - single frame - processed

Image Sets

1. Unaligned multi-frame micrographs of SARS-CoV-2 5' stem-loops 5-6 RNA.

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

16022

Image size:

(4096, 4096)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(0.741, 1.0)

Details:

These micrographs were collected with a Titan Krios G3i, 300kV and a Falcon 4 detector with a 10eV slit of a Selectris energy filter. Over the collection, the sample was exposed evenly to 60 electrons per angstrom squared with a total exposure time of 4.66 seconds. Desired defocuses ranged from -1 to -2 nm. The exposures are gain corrected.

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2. Extracted particles and orientations for final reconstruction of SARS-CoV-2 5' stem-loops 5-6 RNA.

Category:

picked particles - single frame - processed

Image format:

MRC

No. of images or tilt series:

160535

Image size:

(216, 216)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.482, 1.482)

Details:

Particles extracted from motion-corrected micrographs. The orientations for the final reconstruction can be found in data/HCoV-229E_SL5/particles/P3_J296_particles_exported.cs.

Files:

This entry cannot be downloaded from PDBj.

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We recommend using rsync, aspera, globus, etc.

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