EMPIAR-11818 - Cryo-electron tomogram of mitochondria in cryo-FIB milled mouse embryonic fibroblasts with OPA1 overexpression [258 multi-frame micrographs composed of 4 frames each in TIFF format]

EMPIAR-11818

Cryo-electron tomogram of mitochondria in cryo-FIB milled mouse embryonic fibroblasts with OPA1 overexpression [258 multi-frame micrographs composed of 4 frames each in TIFF format]

Publication:

In situ architecture of Opa1-dependent mitochondrial cristae remodeling

Fry MY, Navarro PP, Hakim P, Ananda VY, Qin X, Landoni JC, Rath S, Inde Z, Lugo CM, Luce BE, Ge Y, McDonald JL, Ali I, Ha LL, Kleinstiver BP, Chan DC, Sarosiek KA, Chao LH

The EMBO journal 43 (2024) 391-413

PMID: 38225406

DOI: 10.1038/s44318-024-00027-2

Related EMDB entry:

EMD-43070 EMD-43068 EMD-43067 EMD-43069

Deposited:

2023-12-07

Released:

2024-06-04

Last modified:

2024-06-04

Imageset size:

8.80 GB

Imageset DOI:

10.6019/EMPIAR-11818

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame movies of tilt-series of cryo-FIB milled Mouse Embryonic Fibroblasts with OPA1 overexpression

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

258

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.28, 1.28)

Details:

Unaligned multi-frame micrographs (EMD-430*/frames/*.tiff) corresponding to 5 tilt series with corresponding gain reference (gain/*dm4) and metadata (mdoc/*.mdoc). Data acquired on a K3 BioQuantum detector with no Volta phase plate.

Acquisition parameters differ for each dataset (named after the corresponding EMDB accession code) and are specified below.

EMD-43067:
67 tilts in the series.

EMD-43068:
63 tilts in the series

EMD-43069:
64 tilts in the series

EMD-43070:
64 tilts in the series

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Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Citations

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

CryoTRANS: predicting high-resolution maps of rare conformations from self-supervised trajectories in cryo-EM.

Fan X, Zhang Q, Zhang H, Zhu J, Ju L, Shi Z, Hu M, Bao C. (2024)

Self-assembled superstructure alleviates air-water interface effect in cryo-EM.

Zheng L, Xu J, Wang W, Gao X, Zhao C, Guo W, Sun L, Cheng H, Meng F, Chen B, Sun W, Jia X, Zhou X, Wu K, Liu Z, Ding F, Liu N, Wang HW, Peng H. (2024)

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