EMPIAR-11815 - Cryo-EM of SARS-CoV-1 5' proximal stem-loop 5 [multiple data sets in TIFF and MRC formats]

EMPIAR-11815

Cryo-EM of SARS-CoV-1 5' proximal stem-loop 5 [multiple data sets in TIFF and MRC formats]

Publication:

Tertiary folds of the SL5 RNA from the 5' proximal region of SARS-CoV-2 and related coronaviruses

Kretsch RC, Xu L, Zheludev IN, Zhou X, Huang R, Nye G, Li S, Zhang K, Chiu W, Das R

Proceedings of the National Academy of Sciences of the United States of America 121 (2024)

PMID: 38427602

DOI: 10.1073/pnas.2320493121

Related EMDB entry:

EMD-42816

Deposited:

2023-11-28

Released:

2024-04-02

Last modified:

2024-04-02

Imageset size:

2.16 TB

Imageset DOI:

10.6019/EMPIAR-11815

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
picked particles - single frame - processed

Image Sets

1. Unaligned multi-frame micrographs of SARS-CoV-1 5' stem-loop 5 RNA

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

10376

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.86, 0.86)

Details:

These micrographs were collected with a Titan Krios G3i, 300kV, using a BioQuantum energy filter with slit size 20eV, and a K3 detector. Over the collection, the sample was exposed evenly to 50 electrons per angstrom squared with a total exposure time of 0.59 seconds. Desired defocuses ranged from -1 to -2 nm. The exposures are not gain corrected, the gain reference can be found at data/SARS-CoV-1_SL5/exposures/K3-18210041 Gain Ref. x1.m1.mrc.

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2. Extracted particles and orientations for final reconstruction of SARS-CoV-1 5' stem-loop 5 RNA.

Category:

picked particles - single frame - processed

Image format:

MRC

No. of images or tilt series:

220917

Image size:

(380, 380)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.86, 0.86)

Details:

Particles extracted from motion-corrected micrographs. The orientations for the final reconstruction can be found in data/SARS-CoV-1_SL5/particles/P18_J107_particles_exported.cs.

Files:

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