EMPIAR-11744 - Cryo-EM SPA datasets of Streptococcus pneumoniae NADPH oxidase F397A mutant with NADPH under turnover conditions [multiple data sets in EER format]

EMPIAR-11744

Cryo-EM SPA datasets of Streptococcus pneumoniae NADPH oxidase F397A mutant with NADPH under turnover conditions [multiple data sets in EER format]

Publication:

Structural and mechanistic insights into Streptococcus pneumoniae NADPH oxidase

Dubach VRA, San Segundo-Acosta P, Murphy BJ

Nature structural & molecular biology (2024)

PMID: 39039317

DOI: 10.1038/s41594-024-01348-w

Related EMDB entry:

EMD-18647

Deposited:

2023-10-16

Released:

2024-11-08

Last modified:

2024-11-08

Imageset size:

16.65 TB

Imageset DOI:

10.6019/EMPIAR-11744

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. FUnaligned mutliframe non-gain-normalized EER movies of SpNOX F397A collected on a Krios G4

Category:

micrographs - multiframe

Image format:

EER

No. of images or tilt series:

21895

Image size:

(4096, 4096)

Pixel type:

BIT

Pixel spacing:

(0.573, 0.573)

Details:

Raw EER movies collected using a Krios G4 with a CFEG, Falcon 4 detector and Selectris X filter.

The EER movies contain 924 frames and the total exposure dose of the sample was 70 e/A2. Magnification used was 215kx with a pixel size of 0.573 Å. Energy slit was set to 10 eV.

Gain reference is provided.

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2. Unaligned mutliframe non-gain-normalized EER movies of SpNOX F397A collected on a Krios G4

Category:

micrographs - multiframe

Image format:

EER

No. of images or tilt series:

9583

Image size:

(4096, 4096)

Pixel type:

BIT

Pixel spacing:

(0.573, 0.573)

Details:

Raw EER movies collected using a Krios G4 with a CFEG, Falcon 4 detector and Selectris X filter.

The EER movies contain 889 frames and the total exposure dose of the sample was 70 e/A2. Magnification used was 215kx with a pixel size of 0.573 Å. Energy slit was set to 10 eV.

Gain reference is provided.

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. Unaligned mutliframe non-gain-normalized EER movies of SpNOX F397A collected on a Krios G4

Category:

micrographs - multiframe

Image format:

EER

No. of images or tilt series:

6332

Image size:

(4096, 4096)

Pixel type:

BIT

Pixel spacing:

(0.573, 0.573)

Details:

Raw EER movies collected using a Krios G4 with a CFEG, Falcon 4 detector and Selectris X filter.

The EER movies contain 1008 frames and the total exposure dose of the sample was 70 e/A2. Magnification used was 215kx with a pixel size of 0.573 Å. Energy slit was set to 10 eV.

Gain reference is provided.

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

4. Unaligned mutliframe non-gain-normalized EER movies of SpNOX F397A collected on a Krios G4

Category:

micrographs - multiframe

Image format:

EER

No. of images or tilt series:

7415

Image size:

(4096, 4096)

Pixel type:

BIT

Pixel spacing:

(0.573, 0.573)

Details:

Raw EER movies collected using a Krios G4 with a CFEG, Falcon 4 detector and Selectris X filter.

The EER movies contain 994 frames and the total exposure dose of the sample was 70 e/A2. Magnification used was 215kx with a pixel size of 0.573 Å. Energy slit was set to 10 eV.

Gain reference is provided.

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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