EMPIAR-11743 - Cryo-EM SPA dataset of stably reduced Streptococcus pneumoniae NADPH oxidase with NADH [28120 multi-frame micrographs composed of 938 frames each in EER format]

EMPIAR-11743

Cryo-EM SPA dataset of stably reduced Streptococcus pneumoniae NADPH oxidase with NADH [28120 multi-frame micrographs composed of 938 frames each in EER format]

Publication:

Structural and mechanistic insights into Streptococcus pneumoniae NADPH oxidase

Dubach VRA, San Segundo-Acosta P, Murphy BJ

Nature structural & molecular biology (2024)

PMID: 39039317

DOI: 10.1038/s41594-024-01348-w

Related EMDB entry:

EMD-18646

Deposited:

2023-10-16

Released:

2025-06-26

Last modified:

2025-06-26

Imageset size:

11.07 TB

Imageset DOI:

10.6019/EMPIAR-11743

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned mutliframe non-gain-normalized EER movies of SpNOX collected on a Krios G4

Category:

micrographs - multiframe

Image format:

EER

No. of images or tilt series:

28120

Image size:

(4096, 4096)

Pixel type:

BIT

Pixel spacing:

(0.573, 0.573)

Details:

Raw EER movies collected using a Krios G4 with a CFEG, Falcon 4 detector and Selectris X filter.

The EER movies contain 938 frames and the total exposure dose of the sample was 70 e/A2. Magnification used was 215kx with a pixel size of 0.573 Å. Energy slit was set to 10 eV.

XML files to generate optics groups are provided as well as the gain reference.

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