EMPIAR-11682 - Sheared F-actin raw data from Structures of the free and capped ends of the actin filament [multiple data sets in TIFF format]

EMPIAR-11682

Sheared F-actin raw data from Structures of the free and capped ends of the actin filament [multiple data sets in TIFF format]

Publication:

Structures of the free and capped ends of the actin filament

Carman PJ, Barrie KR, Rebowski G, Dominguez R

Science (New York, N.Y.) 380 (2023) 1287-1292

PMID: 37228182

DOI: 10.1126/science.adg6812

Related EMDB entry:

EMD-28934

Deposited:

2023-09-05

Released:

2023-09-22

Last modified:

2023-09-22

Imageset size:

7.32 TB

Imageset DOI:

10.6019/EMPIAR-11682

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Dataset 1 movies

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

8760

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.54, 0.54)

Details:

Dataset 1 raw movies (*tiff format). Split into sub-directories with ~3000 movies each. The gain reference used for dataset 1 is located in directory /data/Beckman_10282022_sheared/gainref.

Pixel size = 0.54 A/pix (super-resolution, binned by 2 during motion correction)
Voltage = 300kV
Aberration = 2.7 mm
Dose = 44 e/A^2

Files:

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2. Dataset 2 movies

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

1502

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.54, 0.54)

Details:

Dataset 2 raw movies (*tiff format). The gain reference used for dataset 2 is located in directory /data/Beckman_04192023/gainref.

Pixel size = 0.54 A/pix (super-resolution, binned by 2 during motion correction)
Voltage = 300kV
Aberration = 2.7 mm
Dose = 43.7 e/A^2

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. Dataset 3 movies

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

4372

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.54, 0.54)

Details:

Dataset 3 raw movies (*tiff format). Split into sub-directories with ~2200 movies each. The gain reference used for dataset 3 is located in directory /data/Beckman_04202023/gainref.

Pixel size = 0.54 A/pix (super-resolution, binned by 2 during motion correction)
Voltage = 300kV
Aberration = 2.7 mm
Dose = 43.7 e/A^2

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

4. Dataset 4 movies

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

1947

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.54, 0.54)

Details:

Dataset 4 raw movies (*tiff format). The gain reference used for dataset 4 is located in directory /data/Beckman_04212023/gainref.

Pixel size = 0.54 A/pix (super-resolution, binned by 2 during motion correction)
Voltage = 300kV
Aberration = 2.7 mm
Dose = 43.7 e/A^2

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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