EMPIAR-11621 - Cryo-EM micrographs of BP-ffsy filaments [20808 multi-frame micrographs composed of 40 frames each in TIFF format]

EMPIAR-11621

Cryo-EM micrographs of BP-ffsy filaments [20808 multi-frame micrographs composed of 40 frames each in TIFF format]

Publication:

Cell spheroid creation by transcytotic intercellular gelation

Guo J, Wang F, Huang Y, He H, Tan W, Yi M, Egelman EH, Xu B

Nature nanotechnology (2023)

PMID: 37217766

DOI: 10.1038/s41565-023-01401-7

Related EMDB entry:

EMD-27688 EMD-25714

Deposited:

2023-07-24

Released:

2023-08-11

Last modified:

2023-08-18

Imageset size:

5.38 TB

Imageset DOI:

10.6019/EMPIAR-11621

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. raw micrographs and gain reference of BP-ffsy filaments

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

20808

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.12, 1.12)

Details:

Raw micrographs of BP-ffsy. Those two folders were collected at NCEF on the same microscope in the same week. A grid may be switched hench different folders and gain-reference mrc are included. No rotation or flip is needed when applying the gain-ref.

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Citations

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Kim K, Li H, Clarke OB. (2025)

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Self-supervised learning for generalizable particle picking in cryo-EM micrographs.

Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)

Laser flash melting cryo-EM samples to overcome preferred orientation.

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In situ structure determination of conformationally flexible targets using nextPYP.

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CryoPROS: Correcting misalignment caused by preferred orientation using AI-generated auxiliary particles.

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Few-shot learning for non-vitrified ice segmentation.

Vivas-Lago A, Castaño-Díez D. (2025)

Automated removal of corrupted tilts in cryo-electron tomography.

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Probabilistic single-particle cryo-EM ab initio 3D reconstruction in SIMPLE.

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Reanalysis of cryo-EM data reveals ALK-cytokine assemblies with both 2:1 and 2:2 stoichiometries.

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