EMPIAR-11565 - AP2 bound to MSP2N2 lipid nanodisc [7698 multi-frame micrographs composed of 60 frames each in TIFF format]

EMPIAR-11565

AP2 bound to MSP2N2 lipid nanodisc [7698 multi-frame micrographs composed of 60 frames each in TIFF format]

Publication:

Lipid nanodiscs as a template for high-resolution cryo-EM structures of peripheral membrane proteins

Cannon KS, Sarsam RD, Tedamrongwanish T, Zhang K, Baker RW

Journal of structural biology 215 (2023)

PMID: 37364761

DOI: 10.1016/j.jsb.2023.107989

Related EMDB entry:

EMD-40035

Deposited:

2023-03-07

Released:

2023-07-25

Last modified:

2023-07-25

Imageset size:

2.14 TB

Imageset DOI:

10.6019/EMPIAR-11565

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned movies of the AP2 clathrin adaptor bound to an MSP2N2 nanodisc

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

7698

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.88, 0.88)

Details:

This is a dataset of the AP2 clathrin adaptor bound to an MSP2N2 nanodisc. Included are raw movie frames, associated mdoc files from SerialEM, and a gain reference.

Total movies: 7698
Pixel Size: 0.88 Ang
Magnification = 45000
Dose rate: 14.4 e-/pix/s
Exposure Time: 2.71638 seconds
Total dose: 50.5 e-/A2
Frames: 60
GainReference = CountRef_20220401_2_000_X-2Y-2-0.mrc

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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