EMPIAR-11564 - AP2 bound to MSPE3D1 lipid nanodisc with tyrosine cargo peptide [12075 micrographs in MRC format]

EMPIAR-11564

AP2 bound to MSPE3D1 lipid nanodisc with tyrosine cargo peptide [12075 micrographs in MRC format]

Publication:

Lipid nanodiscs as a template for high-resolution cryo-EM structures of peripheral membrane proteins

Cannon KS, Sarsam RD, Tedamrongwanish T, Zhang K, Baker RW

Journal of structural biology 215 (2023)

PMID: 37364761

DOI: 10.1016/j.jsb.2023.107989

Related EMDB entry:

EMD-16804

Deposited:

2023-03-07

Released:

2023-07-25

Last modified:

2023-07-25

Imageset size:

1.04 TB

Imageset DOI:

10.6019/EMPIAR-11564

Experimental metadata:

Download xml json

Contains:

micrographs - single frame

Image Sets

1. Aligned, dose-weighted micrographs of the AP2 clathrin adaptor bound to an MSPE3D1 nanodisc with embedded tyrosine cargo

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

12075

Image size:

(5760, 4092)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.88, 0.88)

Details:

This is a dataset of the AP2 clathrin adaptor bound to an MSP1E3D1 nanodisc with an embedded cargo peptide. Included are aligned and dose weighted micrographs from cryoSPARC.

Total micrographs: 12,075
Pixel Size: 0.88 Ang
Magnification = 45,000
Dose rate: 12.86 e-/pix/s
Exposure Time: 2.71638 seconds
Total dose: 45.1 e-/A2

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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