EMPIAR-11456 - Cryo-electron tomography of ChAdOx spikes HexaPro mutant [68 tilt series in MRC format]

EMPIAR-11456

Cryo-electron tomography of ChAdOx spikes HexaPro mutant [68 tilt series in MRC format]

Publication:

In situ structures of ChAdOx vaccine products reveal native prefusion spikes improved by HexaPro mutations

Ni T, Menconca L, Zhu Y, Howe A, Radecke J, Zhang P

(---)

Related EMDB entry:

EMD-16403 EMD-16697 EMD-16404

Deposited:

2023-03-10

Released:

2023-04-26

Last modified:

2023-04-26

Imageset size:

240.35 GB

Imageset DOI:

10.6019/EMPIAR-11456

Experimental metadata:

Download xml json

Contains:

tilt series

Image Sets

1. Cryo-electron tomography of ChAdOx spikes HexaPro mutant

Category:

tilt series

Image format:

MRC

No. of images or tilt series:

Image size:

(4092, 5760)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(2.2, 2.2)

Details:

Tilt-series were acquired using ThermoFisher Titan Krios microscopes operated at 300 keV, equipped with a K3 camera and Quantum energy filter in zeros-loss mode. The energy slit width was set to 20 eV. The tilt-series were collected with serialEM using a dose-symmetric scheme, starting from 0° with a 3° tilt increment by a group of 3, with an angular range from -60° to 60°. The accumulated exposure for each tilt-series was ~120 e-/Å2, with a defocus range between -2 and -5 µm.

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Citations

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Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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