EMPIAR-11377 - Translating 70S ribosome [19449 multi-frame micrographs composed of 1295 frames each in EER format]

EMPIAR-11377

Translating 70S ribosome [19449 multi-frame micrographs composed of 1295 frames each in EER format]

Publication:

The translating bacterial ribosome at 1.55 Å resolution by open access cryo-EM

Fromm SA, O'Connor KM, Purdy M, Bhatt PR, Loughran G, Atkins JF, Jomaa A, Mattei S

BioRxiv (---)

DOI: 10.1101/2022.08.30.505838

Related EMDB entry:

EMD-15793

Deposited:

2022-09-09

Released:

2023-01-30

Last modified:

2023-01-30

Imageset size:

7.82 TB

Imageset DOI:

10.6019/EMPIAR-11377

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Translating 70S ribosome

Category:

micrographs - multiframe

Image format:

EER

No. of images or tilt series:

19449

Image size:

(4096, 4096)

Pixel type:

BIT

Pixel spacing:

(0.731, 0.731)

Details:

total dose is 40 e/A^2; 16 exposures per stage shift using beam image shift in SerialEM; movie suffix *_1-1.eer to *_1-16.eer reflects the respective position in the multi-shot exposure pattern. Movies are not gain corrected. Gain reference is provided: 20211220_115912_EER_GainReference.gain

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Citations

High-resolution ab initio reconstruction enables cryo-EM structure determination of small particles

Kim K, Li H, Clarke OB. (2025)

CLEM-Reg: an automated point cloud-based registration algorithm for volume correlative light and electron microscopy.

Krentzel D, Elphick M, Domart MC, Peddie CJ, Laine RF, Shand C, Henriques R, Collinson LM, Jones ML. (2025)

CryoEMNet driven symmetry-aware molecular reconstruction through deep learning enhanced electron microscopy.

Arora S, Pan SH, Kumar S, Singh SK, Chauhan U, Alhalabi W, Arya V, Alsulami BS, Hsu CH, Chui KT, Gupta BB. (2025)

PERC: a suite of software tools for the curation of cryoEM data with application to simulation, modeling and machine learning.

Costa-Gomes B, Greer J, Juraschko N, Parkhurst J, Mirecka J, Famili M, Rangel-Smith C, Strickson O, Lowe A, Basham M, Burnley T. (2025)

Self-supervised learning for generalizable particle picking in cryo-EM micrographs.

Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)

Laser flash melting cryo-EM samples to overcome preferred orientation.

Straub MS, Harder OF, Mowry NJ, Barrass SV, Hruby J, Drabbels M, Lorenz UJ. (2025)

In situ structure determination of conformationally flexible targets using nextPYP.

Liu HF, Zhou Y, Huang Q, Martin J, Bartesaghi A. (2025)

EMICSS: added-value annotations for EMDB entries.

Duraisamy AK, Fonseca N, Kleywegt GJ, Patwardhan A, Morris KL. (2025)

Global conformations of <i>Pichia pastoris</i> complex I are distinguished by the binding of a unique interdomain bridging subunit.

Lee CS, Grba DN, Wright JJ, Ivanov BS, Hirst J. (2025)

Overcoming Preferred Orientation in Cryo-EM With Ultrasonic Excitation During Vitrification

Williams HM, Curtis WA, Haubner M, Hruby J, Drabbels M, Lorenz UJ. (2025)

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