EMPIAR-11278 - Amyloid fibril from the antimicrobial peptide uperin 3.5 [multiple data sets in TIFF, MRC and MRCS formats]

EMPIAR-11278

Amyloid fibril from the antimicrobial peptide uperin 3.5 [multiple data sets in TIFF, MRC and MRCS formats]

Publication:

The Cryo-EM structures of two amphibian antimicrobial cross-β amyloid fibrils

Bücker R, Seuring C, Cazey C, Veith K, García-Alai M, Grünewald K, Landau M

Nature communications 13 (2022)

PMID: 35896552

DOI: 10.1038/s41467-022-32039-z

Related EMDB entry:

EMD-14167

Deposited:

2022-09-20

Released:

2022-11-15

Last modified:

2022-11-15

Imageset size:

7.35 TB

Imageset DOI:

10.6019/EMPIAR-11278

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
micrographs - single frame
picked particles - single frame - unprocessed

Image Sets

1. Unaligned movie frames of uperin 3.5 filaments

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

11857

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.85, 0.85)

Details:

Aligned micrographs and picked particles are available in folders MotionCorr and final_segments, respectively.

Files:

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Depending on the number and size of files to be downloaded, this can take an enormous amount of time. Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.

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Download a list of selected files.

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rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

2. Motion-corrected micrographs of uperin 3.5 filaments

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

11857

Image size:

(5760, 4092)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.85, 0.85)

Details:

Raw movies and picked particles are available in folders Movies and final_segments, respectively.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. Picked particles (helical segments) used for final reconstruction of uperin 3.5 filament

Category:

picked particles - single frame - unprocessed

Image format:

MRCS

No. of images or tilt series:

215020

Image size:

(328, 328)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.85, 0.85)

Details:

Raw movies and aligned micrographs are available in folders Movies and MotionCorr, respectively.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Browse All Files

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

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Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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