EMPIAR-11242 - Movies from 30S-RbfA-RimP-RsmA dataset [4395 multi-frame micrographs composed of 27 frames each in MRCS format]

EMPIAR-11242

Movies from 30S-RbfA-RimP-RsmA dataset [4395 multi-frame micrographs composed of 27 frames each in MRCS format]

Publication:

A conserved rRNA switch is central to decoding site maturation on the small ribosomal subunit.

Schedlbauer A, Iturrioz I, Ochoa-Lizzarralde B, Diercks T, López-Alonso JP, Lavin JL, Kaminishi T, Çapuni R, Dhimole N, De Astigarraga E, Gil-Garton D, Fucini P, Connell SR

Science advances 7 (2021) eabf7547-eabf7547

PMID: 34088665

DOI: 10.1126/sciadv.abf7547

Related EMDB entry:

EMD-11772 EMD-11771

Deposited:

2020-06-17

Released:

2022-11-15

Last modified:

2022-11-15

Imageset size:

3.62 TB

Imageset DOI:

10.6019/EMPIAR-11242

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multiframe movies of E. coli 30S subunit bound by RbfA,RimP, and RsmA

Category:

micrographs - multiframe

Image format:

MRCS

No. of images or tilt series:

4395

Image size:

(4096, 4096)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(1.085, 1.085)

Details:

Falcon III (linear) movies collected at eBIC - Titan Krios (M03, em17171-12) at 300 kV, 1.085 Å/pixel and a total exposure of 42 e-/Å^2

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Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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