EMPIAR-11231 - Single particle cryo-EM of KdpFAB(S162A)C under turnover condition [multiple data sets in TIFF format]

EMPIAR-11231

Single particle cryo-EM of KdpFAB(S162A)C under turnover condition [multiple data sets in TIFF format]

Publication:

Inhibited KdpFABC transitions into an E1 off-cycle state

Silberberg JM, Stock C, Hielkema L, Corey RA, Rheinberger J, Wunnicke D, Dubach VRA, Stansfeld PJ, Hänelt I, Paulino C

eLife 11 (2022)

PMID: 36255052

DOI: 10.7554/elife.80988

Related EMDB entry:

EMD-14918 EMD-14919

Deposited:

2022-09-17

Released:

2022-11-16

Last modified:

2022-11-16

Imageset size:

1.78 TB

Imageset DOI:

10.6019/EMPIAR-11231

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned movie frames of the KdpFAB(S162A)C under turnover conditions in DDM (Dataset 1)

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

1023

Image size:

(3838, 3710)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.012, 1.012)

Details:

Unaligned multi-frame movies with each 60 frames per movie collected at 200 kV in counting mode. Total accumulated electron exposure at specimen level corresponds to 53.3 electrons per Å^2. Movie are not gain corrected and the gain reference is provided.

Files:

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2. Unaligned movie frames of the KdpFAB(S162A)C under turnover conditions in DDM (Dataset 2)

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

4449

Image size:

(3838, 3710)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.012, 1.012)

Details:

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. Unaligned movie frames of the KdpFAB(S162A)C under turnover conditions in DDM (Dataset 3)

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

3696

Image size:

(3838, 3710)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.012, 1.012)

Details:

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Browse All Files

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Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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