EMPIAR-11083 - Nudaurelia capensis omega virus maturation intermediates captured at pH5.6 (insect cell expressed VLPs) [multiple data sets in MRC format]

EMPIAR-11083

Nudaurelia capensis omega virus maturation intermediates captured at pH5.6 (insect cell expressed VLPs) [multiple data sets in MRC format]

Publication:

Decoding virus maturation with cryo-EM structures of intermediates

Castells-Graells R, Hesketh EL, Johnson JE, Ranson NA, Lawson DM, Lomonossoff GP

(---)

Related EMDB entry:

EMD-15209

Deposited:

2022-06-27

Released:

2023-02-13

Last modified:

2023-02-13

Imageset size:

13.70 TB

Imageset DOI:

10.6019/EMPIAR-11083

Experimental metadata:

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Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

MRC movies (*Raw_data/*/*/FoilHole*Fractions.mrc) in this entry were compressed by bzip2 (pbzip2)
by EMPIAR Japan to save space and bandwidth.

Some programs (e.g. RELION >= 4.0.1 and CryoSPARC) can process compressed movies without explicit
decompression. For other programs, movies have to be decompressed beforehand. In this case,
you might want to convert decompressed MRC movies into TIFF to save your local storage.

Original size:   21,058,109,534 kilobytes
Compressed size: 12,988,480,073 kilobytes

Contains:

micrographs - multiframe
micrographs - single frame

Image Sets

1. unaligned movies

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

6952

Image size:

(4096, 4096)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(1.065, 1.065)

Details:

Micrographs are unaligned movies comprised of 39 frames each (accumulating 48.7 electron per squared Angstrom in a 1.0 second exposure)

Files:

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2. unaligned movies

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

9526

Image size:

(4096, 4096)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(1.065, 1.065)

Details:

Micrographs are unaligned movies comprised of 39 frames each (accumulating 50.4 electron per squared Angstrom in a 1.0 second exposure)

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

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3. aligned and dose-weighted micrographs

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

6952

Image size:

(4096, 4096)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.065, 1.065)

Details:

Micrographs are averages of aligned and dose-weighted movies

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

4. aligned and dose-weighted micrographs

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

9526

Image size:

(4096, 4096)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.065, 1.065)

Details:

Micrographs are averages of aligned and dose-weighted movies

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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