EMPIAR-10680 - Structural basis for VIPP1 oligomerization and maintenance of thylakoid membrane integrity [8120 multi-frame micrographs composed of 80 frames each in TIFF format]

EMPIAR-10680

Structural basis for VIPP1 oligomerization and maintenance of thylakoid membrane integrity [8120 multi-frame micrographs composed of 80 frames each in TIFF format]

Publication:

Structural basis for VIPP1 oligomerization and maintenance of thylakoid membrane integrity

Gupta TK, Klumpe S, Gries K, Heinz S, Wietrzynski W, Ohnishi N, Niemeyer J, Spaniol B, Schaffer M, Rast A, Ostermeier M, Strauss M, Plitzko JM, Baumeister W, Rudack T, Sakamoto W, Nickelsen J, Schuller JM, Schroda M, Engel BD

Cell 184 (2021) 3643-3659.e23

PMID: 34166613

DOI: 10.1016/j.cell.2021.05.011

Related EMDB entry:

EMD-12712

Deposited:

2021-02-02

Released:

2021-07-30

Last modified:

2021-07-30

Imageset size:

1.98 TB

Imageset DOI:

10.6019/EMPIAR-10680

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned frames of VIPP1 from K2 operated in counting mode

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

8120

Image size:

(3838, 3710)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.35, 1.35)

Details:

Particle coordinates correspond to initial picking using crYOLO. Micrographs where particles were not detected do not have an associated .box file.
The gain reference is provided in MRC format inside the data/gain/ directory.

Data acquisition details:
Images were acquired on a Titan Krios microscope (FEI, Thermo Fisher Scientific) at 300 kV, using a post-column energy filter (Quantum, Gatan) and a K2 Summit direct electron detector (Gatan) operated in counting mode. For each image, 80 frames were acquired over 8 sec, with a total dose of ~45 e−/Å2 and a calibrated pixel size of 1.35 Å. A total of 8120 images were recorded, using a defocus range of −0.5 to −3.5 μm.

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