EMPIAR-10601 - A lamin A/C variant causing striated muscle disease provides insights into filament organization [2 tilt series in MRC format]

EMPIAR-10601

A lamin A/C variant causing striated muscle disease provides insights into filament organization [2 tilt series in MRC format]

Publication:

A lamin A/C variant causing striated muscle disease provides insights into filament organization

Kronenberg-Tenga R, Tatli M, Eibauer M, Wu W, Shin JY, Bonne G, Worman HJ, Medalia O

Journal of cell science 134 (2021)

PMID: 33536248

DOI: 10.1242/jcs.256156

Related EMDB entry:

EMD-13061 EMD-13056

Deposited:

2020-12-16

Released:

2021-01-08

Last modified:

2021-06-18

Imageset size:

2.70 GB

Imageset DOI:

10.6019/EMPIAR-10601

Experimental metadata:

Download xml json

Contains:

tilt series

Image Sets

1. Tilt-series of LmnaH222P/H222P revealing lamin meshwork in nuclease treated and FIB-milled nucleus

Category:

tilt series

Image format:

MRC

No. of images or tilt series:

Image size:

(3708, 3838)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(2.2, 2.2)

Details:

Both nuclease treated near native and FIB-milled LmnaH222P/H222P tilt-series were acquired at a dose-fractionated of 1 frame per 0.2 seconds, at a magnification of 64 000 with a 0.22 nm pixel size and an angular range of -60° to 60°. Nuclease treated tilt series were acquired with bidirectional scheme and recorded with a 2° increment (accumulating ~140 e-/Å2) at a -6 defocus and aligned in imod. FIB-milled raw tilt series were acquired with dose-symmetric scheme and recorded with a 3° increment (accumulating ~155 e-/Å2) at a -4 defocus.

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