EMPIAR-10572 - Krios G4 apoferritin test with K3/BioQuantum SerialEM BIS 1x1x4 [3472 multi-frame micrographs composed of 76 frames each in TIFF format]

EMPIAR-10572

Krios G4 apoferritin test with K3/BioQuantum SerialEM BIS 1x1x4 [3472 multi-frame micrographs composed of 76 frames each in TIFF format]

Publication:

Cryo-EM Performance Testing of Hardware and Data Acquisition Strategies

Danev R, Yanagisawa H, Kikkawa M

Microscopy (Oxford, England) 1 (2021) 1-2

PMID: 33969878

DOI: 10.1093/jmicro/dfab016

Related EMDB entry:

EMD-30685

Deposited:

2020-11-24

Released:

2020-12-02

Last modified:

2021-07-02

Imageset size:

965.99 GB

Imageset DOI:

10.6019/EMPIAR-10572

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame non-gain-normalized movies in LZW compressed TIFF format

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

3472

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.1997, 0.1997)

Details:

Please see the "dataset_details_apoF_set3_EF.txt" file for details.

To test the overall performance of the Krios G4 at The University of Tokyo, five datasets were collected from the same apoferritin grid in the beginning of July 2020.
This was the fourth dataset in the series.

Sample: mouse heavy chain apoferritin
Grid: UltrAuFoil 300 mesh R1.2/1.3
Cemera: Gatan K3 post a BioQuantum energy filter
Camera mode: super resolution, CDS
TEM mode: EFTEM NanoProbe
Mag: x215k
C2 aperture: 50 um
Spot: 6
Beam diameter: 0.65 um
EF slit: 15 eV
Data collection software: SerialEM
Acquisition method: beam-image shift with beam-tilt compensation
Acquisition pattern: 1 x 1 holes, 4 images around the periphery of the hole
Physical Pixel: 0.3994 A/pix
SuperRes pixel: 0.1997 A/pix
Exposure rate: 3.62 e/pix/s (CDS)
Exposure time: 3.04 s
Total exposure: 69.0 e/A2
Frames: 76
Defocus: 0.2 - 0.8 um
Movies: 3472
Data format: non-gain-normalized LZW compressed TIFFs
Gain reference: "apoF_set3_gain.mrc" prepared from the data
Final particles: 203k
Particles file: "run_data.star"
Optics groups: per-acquisition position
Resolution (with Ewald correction): 1.35 A
Rosenthal-Henderson B-factor: 40.9 A^2

Rado
November 25, 2020

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Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

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