EMPIAR-10508 - Cryo-EM structure of the SpCas9 delta4CE Ternary Complex [2570 multi-frame micrographs composed of 60 frames each in TIFF format]

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EMPIAR-10508

Cryo-EM structure of the SpCas9 delta4CE Ternary Complex [2570 multi-frame micrographs composed of 60 frames each in TIFF format]

Publication:

Comprehensive deletion landscape of CRISPR-Cas9 identifies minimal RNA-guided DNA-binding modules

Shams A, Higgins SA, Fellmann C, Laughlin TG, Oakes BL, Lew R, Kim S, Lukarska M, Arnold M, Staahl BT, Doudna JA, Savage DF

Nature communications 12 (2021)

PMID: 34580310

DOI: 10.1038/s41467-021-25992-8

Related EMDB entry:

EMD-22518

Deposited:

2020-08-27

Released:

2021-10-01

Last modified:

2022-01-31

Imageset size:

1.54 TB

Imageset DOI:

10.6019/EMPIAR-10508

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame, non-gain-corrected frames in LZW-TIFF of SpCas9 delta4CE Ternary Complex

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

2570

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.45, 0.45)

Details:

LZW-TIFF, non-gain-corrected, super-resolution K3 multi-frame movies containing 60 frames with cumulative, uniform exposure of 60 electrons per square Angstrom. Gain-reference provided in frame directory as dm4 format. Final particle positions and orientations provided as run_data.star from RELION-3.1b. Data collected using SerialEM with a 3x3 image-shift pattern and beam tilt compensation, but 9th position was systematically poor and removed yielding 8 image-shift groups as marked by the trailing 4 digit-identifier of each movie file.

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