EMPIAR-10459 - FIB SEM images of a Zebrafish hindbrain macrophage containing 2 Toxoplasma gondii tachizoites [multiple data sets in TIFF format]

EMPIAR-10459

FIB SEM images of a Zebrafish hindbrain macrophage containing 2 Toxoplasma gondii tachizoites [multiple data sets in TIFF format]

Publication:

The zebrafish as a novel model for the in vivo study of Toxoplasma gondii replication and interaction with macrophages

Yoshida N, Domart MC, Peddie CJ, Yakimovich A, Mazon-Moya MJ, Hawkins TA, Collinson L, Mercer J, Frickel EM, Mostowy S

Disease models & mechanisms 13 (2020)

PMID: 32461265

DOI: 10.1242/dmm.043091

Related EMDB entry:

Deposited:

2020-07-01

Released:

2020-10-14

Last modified:

2020-10-14

Imageset size:

1.11 TB

Imageset DOI:

10.6019/EMPIAR-10459

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
micrographs - single frame

Image Sets

1. Aligned FIB image stack of a macrophage containing 2 Toxoplasma Gondii tachizoites

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

3200

Image size:

(3437, 3800)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(50.0, 50.0)

Details:

Images were acquired at 5 nm isotropic resolution throughout the region of interest, using a 4.2 us dwell time. During acquisition the SEM was operated at an accelerating voltage of 1.5 kV with 1.5 nA current.

Files:

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2. Raw FIB image stack of a macrophage containing 2 Toxoplasma Gondii tachizoites - part 1

Category:

micrographs - single frame

Image format:

TIFF

No. of images or tilt series:

2062

Image size:

(12032, 12032)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(50.0, 50.0)

Details:

First half of the raw data. See part 2 for second half. Images were acquired at 5 nm isotropic resolution throughout the region of interest, using a 4.2 us dwell time. During acquisition the SEM was operated at an accelerating voltage of 1.5 kV with 1.5 nA current.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. Raw FIB image stack of a macrophage containing 2 Toxoplasma Gondii tachizoites - part 2

Category:

micrographs - single frame

Image format:

TIFF

No. of images or tilt series:

1963

Image size:

(12032, 12032)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(50.0, 50.0)

Details:

Second half of the raw data. See part 1 for first half. Images were acquired at 5 nm isotropic resolution throughout the region of interest, using a 4.2 us dwell time. During acquisition the SEM was operated at an accelerating voltage of 1.5 kV with 1.5 nA current.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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