EMPIAR-10412 - Soft X-ray Tomography of mock-infected U2OS cells [1 tilt series in MRC format]

EMPIAR-10412

Soft X-ray Tomography of mock-infected U2OS cells [1 tilt series in MRC format]

Publication:

3D Correlative Cryo-Structured Illumination Fluorescence and Soft X-ray Microscopy Elucidates Reovirus Intracellular Release Pathway

Kounatidis I, Stanifer ML, Phillips MA, Paul-Gilloteaux P, Heiligenstein X, Wang H, Okolo CA, Fish TM, Spink MC, Stuart DI, Davis I, Boulant S, Grimes JM, Dobbie IM, Harkiolaki M

Cell 182 (2020) 515-530.e17

PMID: 32610083

DOI: 10.1016/j.cell.2020.05.051

Related EMDB entry:

Deposited:

2020-05-08

Released:

2020-06-30

Last modified:

2022-02-08

Imageset size:

2.33 GB

Imageset DOI:

10.6019/EMPIAR-10412

Experimental metadata:

Download xml json

Contains:

tilt series

Image Sets

1. Tomogram of reovirus mock-infected U2OS cells (Area1)

Category:

tilt series

Image format:

MRC

No. of images or tilt series:

Image size:

(946, 946)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(160.0, 160.0)

Details:

Tomogram of reovirus mock-infected U2OS cells (Area1)
EMPIAR entry 10412: U2OS_Reo_MockInfected_Area1_Reconstruction (S-BIAD17)

Note:
Associated with EMPIAR entry 10413 (adjacent/overlapping FOVs)

Sample characteristics:
Substrate = Quantifoil F1 2/2
Fiducials = Au 250nm nanospheres

Data collection:
Data collected at beamline B24 at Diamond Light source
Data collected 18th Feb 2019
Allocation award ID MX21046

Tilt series:
-65deg to 65deg
0.5deg step
Total frames 261
0.5sec exposure

Microscope make:
Zeiss UltraXRM-S220C

Data collection software:
XRM Controller

Microscope setup:
Energy: 500eV
Magnification = x812.5
FOV = 16microns
pixel size = 16x16nm
Zoneplate = 40nm

Data processing:
Type = Automated
Software = Batchruntomo
Alignment protocol = SIRT

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Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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