EMPIAR-10398 - CryoEM reconstruction of ESCRT-III filament composed of IST1 NTD R16E K27E double mutant [stack of 4556 particles in MRCS format]

EMPIAR-10398

CryoEM reconstruction of ESCRT-III filament composed of IST1 NTD R16E K27E double mutant [stack of 4556 particles in MRCS format]

Publication:

Membrane constriction and thinning by sequential ESCRT-III polymerization

Nguyen HC, Talledge N, McCullough J, Sharma A, Moss FR, Iwasa JH, Vershinin MD, Sundquist WI, Frost A

Nature structural & molecular biology 27 (2020) 392-399

PMID: 32251413

DOI: 10.1038/s41594-020-0404-x

Related EMDB entry:

EMD-20591

Deposited:

2020-04-17

Released:

2020-09-02

Last modified:

2020-09-02

Imageset size:

1.74 GB

Imageset DOI:

10.6019/EMPIAR-10398

Experimental metadata:

Download xml json

Contains:

picked particles - single frame - processed

Image Sets

1. CryoEM data of ESCRT-III filament composed of IST1 NTD R16E K27E double mutant

Category:

picked particles - single frame - processed

Image format:

MRCS

No. of images or tilt series:

4556

Image size:

(320, 320)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.23, 1.23)

Details:

particles used to reconstruct an IST1 double mutant alone using helical reconstruction in RELION

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Citations

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Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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