EMPIAR-10361 - Cryo-EM of CRF1 receptor bound to CRF and Gs protein [4300 multi-frame micrographs composed of 62 frames each in TIFF format]

EMPIAR-10361

Cryo-EM of CRF1 receptor bound to CRF and Gs protein [4300 multi-frame micrographs composed of 62 frames each in TIFF format]

Publication:

Toward a Structural Understanding of Class B GPCR Peptide Binding and Activation

Liang YL, Belousoff MJ, Zhao P, Koole C, Fletcher MM, Truong TT, Julita V, Christopoulos G, Xu HE, Zhang Y, Khoshouei M, Christopoulos A, Danev R, Sexton PM, Wootten D

Molecular cell 77 (2020) 656-668.e5

PMID: 32004469

DOI: 10.1016/j.molcel.2020.01.012

Related EMDB entry:

EMD-30003 EMD-20277

Deposited:

2020-02-10

Released:

2020-03-20

Last modified:

2020-02-13

Imageset size:

2.64 TB

Imageset DOI:

10.6019/EMPIAR-10361

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame gain-normalized movies in LZW compressed TIFF format

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

4300

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.83, 0.83)

Details:

- Most of the movies were collected with the Volta phase plate (VPP). Such movies are in the "VPP" subfolder and contain "VPP" in their filename.
- The movies were collected with a 9-hole beam-image shift acquisition pattern. The filename contains a four-digit number in the range 0000 to 0008 corresponding to the image-shift acquisition position.
- The image-shift induced beam-tilt was not compensated optically during acquisition. For best results, perform per-image-shift-position beam-tilt measurement and correction with CTF refinement.
- The movies were recorded with Gatan K3 camera in counting (hardware bin by 2) mode and saved as gain-normalized LZW compressed TIFF files by SerialEM.

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Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

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