EMPIAR-10249
Horse liver alcohol dehydrogenase movies obtained using Talos Arctica operating at 200 kV equipped with a K2 [1153 multi-frame micrographs composed of 44 frames each in MRC format]
Publication:

High-resolution structure determination of sub-100 kilodalton complexes using conventional cryo-EM

Herzik Jr MA, Wu M, Lander GC

bioRxiv (2018)

Related EMDB entry:
Deposited:
2018-12-10
Released:
2019-03-18
Last modified:
2019-03-18
Imageset size:
146.16 GB
Imageset DOI:
Experimental metadata:
Download xml json

Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

MRC movies (*frames.mrc) in this entry were compressed by bzip2 (pbzip2)
by EMPIAR Japan to save space and bandwidth.

Some programs (e.g. RELION >= 4.0.1 and CryoSPARC) can process compressed movies without explicit
decompression. For other programs, movies have to be decompressed beforehand. In this case,
you might want to convert decompressed MRC movies into TIFF to save your local storage.

The original entry contained one temporary file (17aug05a/rawdata/17aug05a_00016hl16_00003edhiii.frames.tif.tmp),
which was removed.

Original size:   1,408,437,840 kilobytes
Compressed size:   153,147,141 kilobytes
Contains:
  • micrographs - multiframe
1. Raw, unaligned movies of horse liver alcohol dehydrogenase acquired on a Talos Arctica using a K2 direct electron detector
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
1153
Image size:
(3710, 3838)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(0.5585, 0.5585)
Details:
Raw, unaligned counting mode frame stacks (44 frames) of horse liver alcohol dehydrogenase in vitreous ice imaged on a Talos Arctica using a K2 direct electron detector. Two separate data collections that were combined are provided. Images used for corrections are also included (require flip in Y).
Files:
Loading...
Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)
Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)
Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)
Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)
Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)