EMPIAR-10244 - Cryo electron micrographs of RNA polymerase II transcribing a nucleosome [2719 multi-frame micrographs composed of 40 frames each in TIFF format]

EMPIAR-10244

Cryo electron micrographs of RNA polymerase II transcribing a nucleosome [2719 multi-frame micrographs composed of 40 frames each in TIFF format]

Publication:

Structural basis of the nucleosome transition during RNA polymerase II passage

Kujirai T, Ehara H, Fujino Y, Shirouzu M, Sekine S, Kurumizaka H

Science (New York, N.Y.) 362 (2018) 595-598

PMID: 30287617

DOI: 10.1126/science.aau9904

Related EMDB entry:

EMD-6982

Deposited:

2019-01-24

Released:

2019-02-01

Last modified:

2019-02-01

Imageset size:

1.36 TB

Imageset DOI:

10.6019/EMPIAR-10244

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned movies of RNA polymerase II transcribing a nucleosome

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

2719

Image size:

(7420, 7676)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.745, 0.745)

Details:

Unaligned movies in compressed TIFF (collected on 4 sessions).

The following parameters were used for MotionCor2:
-Bft 150 -PixSize 0.745 -Patch 5 5 -Gain SuperRef_XXXX.mrc -FtBin 2 -Kv 200 -FmDose 1.25 -Iter 10 -Throw 2 -RotGain 1 -FlipGain 2

Files:

Archives and downloads the selected files into an uncompressed zip file.

Depending on the number and size of files to be downloaded, this can take an enormous amount of time. Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Browse All Files

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Quick Links

Talks and Tutorials

EMPIAR Quick tour

Statistics

Publications

Re-use case study

EMPIAR in the news

Claim entries to your ORCiD

COVID-19 Data Portal

EMDB

PDBe

BioImage Archive

EMNavigator@PDBj

PDBj

RCSB@PDBj

EMDataResoruce@RCSB,EMDB,NCMI

Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

See all citations

Protein Data Bank Japan News

[2024-11-01]

A new portal integrating genome variants and protein structures has released

[2024-10-30]

294 new PDB entries have been released on 2024-10-30.

[2024-10-25]

PDBj luncheon seminar at APBJC 2024

[2024-10-17]

[wwPDB] The Nobel Prize in Chemistry 2024

[2024-10-16]

[wwPDB] wwPDB Data Release Policy on Public Preprint Archives