EMPIAR-10244 - Cryo electron micrographs of RNA polymerase II transcribing a nucleosome [2719 multi-frame micrographs composed of 40 frames each in TIFF format]

EMPIAR-10244

Cryo electron micrographs of RNA polymerase II transcribing a nucleosome [2719 multi-frame micrographs composed of 40 frames each in TIFF format]

Publication:

Structural basis of the nucleosome transition during RNA polymerase II passage

Kujirai T, Ehara H, Fujino Y, Shirouzu M, Sekine S, Kurumizaka H

Science (New York, N.Y.) 362 (2018) 595-598

PMID: 30287617

DOI: 10.1126/science.aau9904

Related EMDB entry:

EMD-6982

Deposited:

2019-01-24

Released:

2019-02-01

Last modified:

2019-02-01

Imageset size:

1.36 TB

Imageset DOI:

10.6019/EMPIAR-10244

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned movies of RNA polymerase II transcribing a nucleosome

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

2719

Image size:

(7420, 7676)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.745, 0.745)

Details:

Unaligned movies in compressed TIFF (collected on 4 sessions).

The following parameters were used for MotionCor2:
-Bft 150 -PixSize 0.745 -Patch 5 5 -Gain SuperRef_XXXX.mrc -FtBin 2 -Kv 200 -FmDose 1.25 -Iter 10 -Throw 2 -RotGain 1 -FlipGain 2

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