EMPIAR-10178 - Single particle cryo-EM dataset of the flexible and variable oligomeric state complex AP-1:Arf1:tetherin-HIV-Nef [stack of 53841 particles in MRCS format]

EMPIAR-10178

Single particle cryo-EM dataset of the flexible and variable oligomeric state complex AP-1:Arf1:tetherin-HIV-Nef [stack of 53841 particles in MRCS format]

Publication:

HIV-1 Nefs Are Cargo-Sensitive AP-1 Trimerization Switches in Tetherin Downregulation

Sturzel CM, Heusinger E, Kirchhoff F, Ren X, Hurley JH

Cell 174 (2018) 659-671.e14

PMID: 30053425

DOI: 10.1016/j.cell.2018.07.004

Related EMDB entry:

EMD-7457 EMD-7456 EMD-7455 EMD-7458

Deposited:

2018-03-10

Released:

2018-07-27

Last modified:

2018-07-27

Imageset size:

10.09 GB

Imageset DOI:

10.6019/EMPIAR-10178

Experimental metadata:

Download xml json

Contains:

picked particles - multiframe - processed

Image Sets

1. Dose weight particle stack of AP1:Arf1:tetherin-HIV-Nef closed trimer after monomeric subunit extraction using LocalRec

Category:

picked particles - multiframe - processed

Image format:

MRCS

No. of images or tilt series:

53841

Image size:

(224, 224)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.067, 1.067)

Details:

Particles of the monomeric subunit of the AP1:Arf1:tetherin-HIV-Nef closed trimer extracted by LocalRec and including signal subtraction to leave only density for the monomeric subunit of interest.

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Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

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