EMPIAR-10176 - Single particle cryo-EM dataset of the flexible and variable oligomeric state complex AP-1:Arf1:tetherin-HIV-Nef [stack of 209816 particles in MRCS format]

EMPIAR-10176

Single particle cryo-EM dataset of the flexible and variable oligomeric state complex AP-1:Arf1:tetherin-HIV-Nef [stack of 209816 particles in MRCS format]

Publication:

HIV-1 Nefs Are Cargo-Sensitive AP-1 Trimerization Switches in Tetherin Downregulation

Morris KL, Buffalo CZ, Stürzel CM, Heusinger E, Kirchhoff F, Ren X, Hurley JH

Cell 174 (2018) 659-671.e14

PMID: 30053425

DOI: 10.1016/j.cell.2018.07.004

Related EMDB entry:

EMD-7457 EMD-7456 EMD-7455 EMD-7458

Deposited:

2018-03-06

Released:

2018-07-27

Last modified:

2018-07-27

Imageset size:

115.37 GB

Imageset DOI:

10.6019/EMPIAR-10176

Experimental metadata:

Download xml json

Contains:

picked particles - multiframe - unprocessed

Image Sets

1. Dose weighted particle stack of AP1:Arf1:tetherin-HIV-Nef

Category:

picked particles - multiframe - unprocessed

Image format:

MRCS

No. of images or tilt series:

209816

Image size:

(384, 384)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.067, 1.067)

Details:

Picked particles after 2D class averaging in Relion to clean the dataset. 209,816 particles represent the AP1:Arf1:tetherin-Nef dataset before 3D classification of the multiple oligomeric and flexible states of the complex.

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Citations

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Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

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Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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