EMPIAR-12876 - The CryoEM structure of N49-P9.6-FR3 and PGT121 Fabs in complex with BG505 SOSIP.664 [multiple data sets in TIFF format]

EMPIAR-12876

The CryoEM structure of N49-P9.6-FR3 and PGT121 Fabs in complex with BG505 SOSIP.664 [multiple data sets in TIFF format]

Publication:

A comprehensive engineering strategy improves potency and manufacturability of a near pan-neutralizing antibody against HIV

Sajadi MM, Abbasi A, Tehrani ZR, Siska C, Clark R, Chi W, Seaman MS, Mielke D, Wagh K, Liu Q, Jumpa T, Ketchem RR, Nguyen DN, Tolbert WD, Pierce BG, Atkinson B, Deming D, Sprague M, Asakawa A, Ferrer D, Dunn Y, Calvillo S, Yin R, Guest JD, Korber B, Mayer BT, Sato AH, Ouyang X, Foulke S, Habibzadeh P, Karimi M, Aslanabadi A, Hojabri M, Saadat S, Zareidoodeji R, Kędzior M, Pozharski E, Heredia A, Chen H, Montefiori D, Ferrari G, Pazgier M, Lewis GK, Jardine JG, Lusso P, DeVico A

Structure (London, England : 1993) (2025)

PMID: 40373766

DOI: 10.1016/j.str.2025.04.016

Related EMDB entry:

EMD-26648

Deposited:

2025-06-09

Released:

2025-07-18

Last modified:

2025-07-18

Imageset size:

702.00 GB

Imageset DOI:

10.6019/EMPIAR-12876

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Grid R374_S1512 set 1 in bzip2 compressed TIFF format

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

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(None, None)

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UNSIGNED BYTE

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Files are compressed with bzip2
Voltage = 200
GainReference = CountRef_R374_S1512_127-1_000.mrc
ExposureDose = 54.9633
DoseRate = 11.4175
PixelSpacing = 0.8893
ExposureTime = 3.99867
NumSubFrames = 42
FrameDosesAndNumber = 1.3086 42

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2. Grid R374_S1512 set 2 in bzip2 compressed TIFF format

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

Image size:

(None, None)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.8893, 0.8893)

Details:

Files are compressed with bzip2.
Voltage = 200
GainReference = CountRef_R374_S1512_143-1_000.mrc
ExposureDose = 54.9633
DoseRate = 9.49503
PixelSpacing = 0.8893
ExposureTime = 3.99867
NumSubFrames = 42
FrameDosesAndNumber = 1.3086 42

Files:

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3. Grid R374_S1512 set 3 in bzip2 compressed TIFF format

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

168

Image size:

(None, None)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.8893, 0.8893)

Details:

Files are compressed bzip2.
Voltage = 200
GainReference = CountRef_R374_S1512_153-1_000.mrc
ExposureDose = 54.9633
DoseRate = 11.1836
PixelSpacing = 0.8893
ExposureTime = 3.99867
NumSubFrames = 42
FrameDosesAndNumber = 1.3086 42

Files:

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4. Grid R374_S1512 set 4 in bzip2 compressed TIFF format

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

Image size:

(None, None)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.8893, 0.8893)

Details:

Files are compressed with bzip2.
Voltage = 200
GainReference = CountRef_R374_S1512_171-1_000.mrc
ExposureDose = 54.9633
DoseRate = 11.387
PixelSpacing = 0.8893
ExposureTime = 3.99867
NumSubFrames = 42
FrameDosesAndNumber = 1.3086 42

Files:

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5. Grid R374_S1512 set 5 in bzip2 compressed TIFF format

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

635

Image size:

(None, None)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.8893, 0.8893)

Details:

Files are compressed with bzip2.
Voltage = 200
GainReference = CountRef_R374_S1512_180-1_000.mrc
ExposureDose = 54.9633
DoseRate = 11.1621
PixelSpacing = 0.8893
ExposureTime = 3.99867
NumSubFrames = 42
FrameDosesAndNumber = 1.3086 42

Files:

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6. Grid R375_S1514 set 1 in bzip2 compressed TIFF format

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

474

Image size:

(None, None)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.8893, 0.8893)

Details:

Files are compressed with bzip2.
Voltage = 200
GainReference = CountRef_R375_S1714_163-1_000_X-1Y-1-0.mrc
ExposureDose = 58.8084
DoseRate = 11.3862
PixelSpacing = 0.8893
NumSubFrames = 45
FrameDosesAndNumber = 1.3069 45

Files:

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7. Grid R375_S1514 set 2 in bzip2 compressed TIFF format

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

1111

Image size:

(None, None)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.8893, 0.8893)

Details:

Files are compressed with bzip2.
Voltage = 200
GainReference = CountRef_R375_S1714_182-2_000_X-1Y-1-0.mrc
ExposureDose = 58.8084
DoseRate = 11.2212
PixelSpacing = 0.8893
ExposureTime = 4.28429
NumSubFrames = 45
FrameDosesAndNumber = 1.3069 45

Files:

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