EMPIAR-12799 - CryoEM dataset of Retron-Eco1 "-1-turn" mutant filaments in the presence of NAD+ [28871 multi-frame micrographs composed of 40 frames each in EER format]

EMPIAR-12799

CryoEM dataset of Retron-Eco1 "-1-turn" mutant filaments in the presence of NAD+ [28871 multi-frame micrographs composed of 40 frames each in EER format]

Publication:

Retron-Eco1 assembles NAD⁺-hydrolyzing filaments that provide immunity against bacteriophages

Carabias A, Camara-Wilpert S, Mestre MR, Lopéz-Méndez B, Hendriks IA, Zhao R, Pape T, Fuglsang A, Luk SH, Nielsen ML, Pinilla-Redondo R, Montoya G

Molecular cell 84 (2024) 2185-2202000000000000.0

PMID: 38788717

DOI: 10.1016/j.molcel.2024.05.001

Related EMDB entry:

EMD-19792

Deposited:

2025-05-26

Released:

2025-06-30

Last modified:

2025-06-30

Imageset size:

12.77 TB

Imageset DOI:

10.6019/EMPIAR-12799

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multiframe micrographs of Retron-Eco1 "-1 turn" mutant

Category:

micrographs - multiframe

Image format:

EER

No. of images or tilt series:

28871

Image size:

(4096, 4096)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.832, 0.832)

Details:

Movies were collected in a Titan Krios G2 equipped with a TFS Falcon III camera operated at 300 keV in counting mode at a magnification of x 165000 (0.725 A˚ /px), using a total dose of 50 e/A˚ 2. Spherical Aberration: 2.7 mm. Out of the original 28871 micrographs, 26559 were used for particle picking. Micrographs are non gain-corrected, non-motion-corrected. The gain reference file is located in the Data folder, file: "20231221_154105_EER_GainReference.gain". Up sampling factor: 1 was used for the processing in CryoSPARC.

Microscope Settings:
-Acceleration Voltage: 300 kV
-Pixel Size: 0.725 Å
-Spherical Aberration: 2.7 mm
-Total Exposure Dose: 50 e/Å²

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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