EMPIAR-12742 - CryoEM movies of human methemoglobin (metHb) under Al's Oil [multiple data sets in EER and MRC formats]

EMPIAR-12742

CryoEM movies of human methemoglobin (metHb) under Al's Oil [multiple data sets in EER and MRC formats]

Publication:

Preparation of oxygen-sensitive proteins for high-resolution cryoEM structure determination using blot-free vitrification

Cook BD, Narehood SM, McGuire KL, Li Y, Akif Tezcan F, Herzik Jr MA

Nature communications 16 (2025) 3528-3528

PMID: 40229244

DOI: 10.1038/s41467-025-58243-1

Related EMDB entry:

EMD-45819 EMD-45820

Deposited:

2025-04-25

Released:

2025-06-26

Last modified:

2025-06-26

Imageset size:

1.44 TB

Imageset DOI:

10.6019/EMPIAR-12742

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
micrographs - single frame

Image Sets

1. Unaligned multi-frame micrographs of human methemoglobin (metHb) under Al's Oil

Category:

micrographs - multiframe

Image format:

EER

No. of images or tilt series:

2072

Image size:

(4096, 4096)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.735, 0.735)

Details:

Unaligned multi-frame micrographs of human methemoglobin (metHb) under Al's Oil collected on a Titan Krios G4 operating at 300 kV equipped with a Selectris-X energy filter with a 10eV slit width. All images were collected at a nominal magnification of 165,000x in EF-TEM mode (with a calibrated pixel size of 0.735 Å) on a Falcon4 direct electron detector. Micrographs were acquired in EER format with a cumulative electron exposure of 60 electrons/Å2.

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2. Aligned micrographs of human methemoglobin (metHb) under Al's Oil

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

2072

Image size:

(4096, 4096)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.735, 0.735)

Details:

Aligned micrographs of human methemoglobin (metHb) under Al's Oil collected on a Titan Krios G4 operating at 300 kV equipped with a Selectris-X energy filter with a 10eV slit width. All images were collected at a nominal magnification of 165,000x in EF-TEM mode (with a calibrated pixel size of 0.735 Å) on a Falcon4 direct electron detector. Micrographs were acquired in EER format with a cumulative electron exposure of 60 electrons/Å2.

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. Gain file for motion correction of unaligned micrographs of human methemoglobin (metHb) under Al's Oil

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

Image size:

(4096, 4096)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.735, 0.735)

Details:

Gain file required for motion correction of unaligned micrographs of human methemoglobin (metHb) under Al's Oil.

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

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Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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