EMPIAR-12711 - Structural insights into transcriptional regulation by the helicase RECQL5 [6190 multi-frame micrographs composed of 40 frames each in TIFF format]

EMPIAR-12711

Structural insights into transcriptional regulation by the helicase RECQL5 [6190 multi-frame micrographs composed of 40 frames each in TIFF format]

Publication:

Structural insights into transcriptional regulation by the helicase RECQL5

Ariza AJF, Lue NZ, Grob P, Kaeser B, Fang J, Kassube SA, Nogales E

bioRxiv (2025)

PMID: 39975028

DOI: 10.1101/2025.01.29.634372

Related EMDB entry:

EMD-48073 EMD-48071 EMD-48074

Deposited:

2025-04-15

Released:

2025-05-09

Last modified:

2025-05-09

Imageset size:

2.79 TB

Imageset DOI:

10.6019/EMPIAR-12711

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of human RNA Polymerase II elongation complex bound to the RECQL5 helicase in the absence of nucleotide

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

6190

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.525, 0.525)

Details:

This dataset contains raw, uncorrected movies collected in super-resolution mode (collected using a Titan Krios G3i, 300 kV, spherical aberration 2.7 mm, total dose 50 e/A^2). The super-resolution pixel size is 0.525 A. The directory also contains the gain reference file (SuperCDSRef_230615_RecQ_230615_Navigator_104_0001_X+0Y+0-1.mrc). This should not be rotated or flipped for processing in cryoSPARC.

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