EMPIAR-12595 - Cryo-EM of RNAP post-terminal complexes bound to RapA+ADP+AlF3 on negatively supercoiled DNA [multiple data sets in TIFF format]

EMPIAR-12595

Cryo-EM of RNAP post-terminal complexes bound to RapA+ADP+AlF3 on negatively supercoiled DNA [multiple data sets in TIFF format]

Publication:

RapA opens the RNA polymerase clamp to disrupt post-termination complexes and prevent cytotoxic R-loop formation

Brewer JJ, Inlow K, Mooney RA, Bosch B, Olinares PDB, Marcelino LP, Chait BT, Landick R, Gelles J, Campbell EA, Darst SA

Nature structural & molecular biology (2025)

PMID: 39779919

DOI: 10.1038/s41594-024-01447-8

Related EMDB entry:

EMD-40943

Deposited:

2025-02-28

Released:

2025-04-09

Last modified:

2025-04-09

Imageset size:

1.67 TB

Imageset DOI:

10.6019/EMPIAR-12595

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned raw movies

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

20770

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.076, 1.076)

Details:

FEI Titan Krios + Gatan K3, counting mode, BioQuantum energy filter 20 eV slit, Cs 2.7 mm; accelerating voltage 300 kV; dose rate 28.40 e-/ A^2/s, frame time 0.05 s (38 frames) with total exposure of 1.90 s, total dose of 53.95 e-/A^2, pixel size 1.076 A/px; defocus range -0.9 to -2.5 um; gain reference is 22jun18a_18113825_01_4092x5760_norm_0.mrc (see references folder).

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2. Unaligned raw movies

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

20770

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.844, 0.844)

Details:

FEI Titan Krios + Gatan K3, counting mode, BioQuantum energy filter 20 eV slit, Cs 2.7 mm; accelerating voltage 300 kV; dose rate 28.40 e-/ A^2/s, frame time 0.05 s (38 frames) with total exposure of 1.90 s, total dose of 53.95 e-/A^2, pixel size 0.844 A/px(detector); pixel size 0.422 A/px(image); defocus range -0.9 to -2.5 um; gain reference is 22jun18a_18113825_01_4092x5760_norm_0.mrc (see references folder).

Files:

This entry cannot be downloaded from PDBj.

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We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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