EMPIAR-12415 - CryoEM of E. coli RNA polymerase and lambda PR promoter DNA 120 ms after mixing [multiple data sets in TIFF format]

EMPIAR-12415

CryoEM of E. coli RNA polymerase and lambda PR promoter DNA 120 ms after mixing [multiple data sets in TIFF format]

Publication:

Early intermediates in bacterial RNA polymerase promoter melting visualized by time-resolved cryo-electron microscopy

Saecker RM, Mueller AU, Malone B, Chen J, Budell WC, Dandey VP, Maruthi K, Mendez JH, Molina N, Eng ET, Yen LY, Potter CS, Carragher B, Darst SA

Nature structural & molecular biology (2024)

PMID: 38951624

DOI: 10.1038/s41594-024-01349-9

Related EMDB entry:

EMD-41437 EMD-41433 EMD-41439 EMD-41448

Deposited:

2024-10-04

Released:

2025-01-17

Last modified:

2025-01-17

Imageset size:

6.09 TB

Imageset DOI:

10.6019/EMPIAR-12415

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned raw movies

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

2707

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.844, 0.844)

Details:

FEI Titan Krios + Gatan K3, counting mode, BioQuantum energy filter 20 eV slit, Cs 2.7 mm; accelerating voltage 300 kV; dose rate 13.81 e-/ A^2/s, frame time 0.1 s (25 frames) with total exposure of 2.5 s, total dose of 34.52 e-/A^2; pixel size 0.844 A/px(detector); pixel size 0.422 A/px(image); defocus range -1.0 to -2.3 um; gain reference is 20feb14f_14112702_01_4092x5760_norm_0.mrc.

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2. Unaligned raw moves

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

2637

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.844, 0.844)

Details:

FEI Titan Krios + Gatan K3, counting mode, BioQuantum energy filter 20 eV slit, Cs 2.7 mm; accelerating voltage 300 kV; dose rate 22.45 e-/ A^2/s, frame time 0.1 s (25 frames) with total exposure of 2.5 s, total dose of 56.13e-/A^2; pixel size 0.844 A/px(detector); pixel size 0.422 A/px(image); defocus range 0 to -2.5 um; gain reference is 20feb14f_14112702_01_4092x5760_norm_0.mrc.

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

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3. Unaligned raw movies

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

21634

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.844, 0.844)

Details:

FEI Titan Krios + Gatan K3, counting mode, BioQuantum energy filter 20 eV slit, Cs 2.7 mm; accelerating voltage 300 kV; dose rate 33.68 e-/ A^2/s, frame time 0.03 s (60 frames) with total exposure of 1.8 s; total dose of 60.63 e-/A^2; pixel size 0.844 A/px(detector); pixel size 0.422 A/px(image); defocus range -1.2 to -2.5 um; gain reference is 21jun11d_11144945_13_4092x5760_norm_0.mrc

Files:

This entry cannot be downloaded from PDBj.

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

See all citations

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