EMPIAR-12301 - Cryo-EM structure of Aquifex aeolicus RseP (wild-type) in complex with 4A9 Fab [11400 multi-frame micrographs composed of 64 frames each in TIFF format]

EMPIAR-12301

Cryo-EM structure of Aquifex aeolicus RseP (wild-type) in complex with 4A9 Fab [11400 multi-frame micrographs composed of 64 frames each in TIFF format]

Publication:

Cryo-EM structure of the bacterial intramembrane metalloprotease RseP in the substrate-bound state

Asahi K, Hirose M, Aruga R, Shimizu Y, Tajiri M, Tanaka T, Adachi Y, Tanaka Y, Kaneko MK, Kato Y, Akashi S, Akiyama Y, Hizukuri Y, Kato T, Nogi T

Science advances 11 (2025)

PMID: 40009668

DOI: 10.1126/sciadv.adu0925

Related EMDB entry:

EMD-61213

Deposited:

2024-09-18

Released:

2025-06-19

Last modified:

2025-06-19

Imageset size:

2.72 TB

Imageset DOI:

10.6019/EMPIAR-12301

Experimental metadata:

Download xml json

Deposited via PDBj

Contains:

micrographs - multiframe

Image Sets

1. Cryo-EM structure of Aquifex aeolicus RseP (wild-type) in complex with 4A9 Fab

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

11400

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.675, 0.675)

Details:

- Acceleration voltage (kV): 300
- Pixel size (A/pixel): 0.675
- Spherical aberration (Cs) : 0.019 mm
- Average electron dose per image (e-/A2): 60
- Number of frames per image: 64
- Please use 'gain.mrc' as the gain reference with the y-axis flipped and no rotation.

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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