EMPIAR-12161 - SBF SEM images of a notoroid in transverse orientation (Fig S24) [multiple data sets in DM4 and TIFF formats]

EMPIAR-12161

SBF SEM images of a notoroid in transverse orientation (Fig S24) [multiple data sets in DM4 and TIFF formats]

Publication:

Notochord and axial progenitor generation by timely BMP and NODAL inhibition during vertebrate trunk formation

Tiago Rito TR, Ashley R.G. Libby ARGL, Madeleine Demuth MD, Marie-Charlotte Domart MCD, Jake Cornwall-Scoones JCS, James Briscoe JB

(---)

DOI: 10.1038/s41586-024-08332-w

Related EMDB entry:

Deposited:

2024-02-14

Released:

2024-09-26

Last modified:

2024-09-26

Imageset size:

12.40 GB

Imageset DOI:

10.6019/EMPIAR-12161

Experimental metadata:

Download xml json

Contains:

micrographs - single frame

Image Sets

1. Low magnification raw SBF SEM overview images (ROI00) of notoroid in transverse orientation

Category:

micrographs - single frame

Image format:

DM4

No. of images or tilt series:

Image size:

(7000, 7000)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(610.0, 610.0)

Details:

Low magnification overview SBF SEM images (ROI00), linked to higher magnification images in ROI01 and ROI02 below. Imaged at 1.8kV, 2us dwell, with FCC with a 30um aperture. 50nm slices.

Files:

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2. High magnification raw SBF SEM images (ROI01) of notochord in notoroid in transverse orientation

Category:

micrographs - single frame

Image format:

DM4

No. of images or tilt series:

Image size:

(7000, 7000)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(88.0, 88.0)

Details:

High magnification images (ROI01) linked to lower magnification images in ROI00. Imaged at 1.8kV, 2us dwell, with FCC with a 30um aperture. 50nm slices.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. High magnification raw SBF SEM images (ROI02) of notochord in notoroid in transverse orientation

Category:

micrographs - single frame

Image format:

DM4

No. of images or tilt series:

Image size:

(7000, 7000)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(83.0, 83.0)

Details:

High magnification images (ROI02) linked to lower magnification images in ROI00. Imaged at 1.8kV, 2us dwell, with FCC with a 30um aperture. 50nm slices.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

4. Survey image showing the relative position of each of the ROIs above

Category:

micrographs - single frame

Image format:

TIFF

No. of images or tilt series:

Image size:

(None, None)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(None, None)

Details:

Survey image used to setup the multi ROI run in Digital Micrograph. Shows the position of each ROI.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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