EMPIAR-12070 - Hypopseudouridylated yeast 80S bound with Taura syndrome virus (TSV) internal ribosome entry site (IRES) [ three datasets, in TIF format] [multiple data sets in TIFF format]

EMPIAR-12070

Hypopseudouridylated yeast 80S bound with Taura syndrome virus (TSV) internal ribosome entry site (IRES) [ three datasets, in TIF format] [multiple data sets in TIFF format]

Publication:

Regulation of translation by ribosomal RNA pseudouridylation

Zhao Y, Rai J, Li H

Science advances 9 (2023)

PMID: 37595043

DOI: 10.1126/sciadv.adg8190

Related EMDB entry:

EMD-28642 EMD-28632 EMD-28633 EMD-28636 EMD-28643 EMD-28634 EMD-28635 EMD-28610

Deposited:

2024-04-17

Released:

2024-06-16

Last modified:

2024-06-16

Imageset size:

16.00 TB

Imageset DOI:

10.6019/EMPIAR-12070

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unalinged multi-frame micrographs of Hypopseudouridylated yeast 80S bound with TSV-IRES RNA [initiation complex]

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

3878

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.53, 0.53)

Details:

Gain ref is save in data/Initiation_Complex/gain/ folder.
Total dose 60 e.
no rotation, Flip upsidedown for motioncor in RELION; no rotation, no flip in CryoSPARC.

Files:

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2. Unalinged multi-frame micrographs of Hypopseudouridylated yeast 80S bound with TSV-IRES RNA [sordarin bound translocation complex]

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

6082

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.53, 0.53)

Details:

Gain ref is save in data/Initiation_Complex/gain/ folder.
Total dose 60 e.
no rotation, Flip upsidedown for motioncor in RELION; no rotation, no flip in CryoSPARC.

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. Unalinged multi-frame micrographs of Hypopseudouridylated yeast 80S bound with TSV-IRES RNA [termination complex]

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

16443

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.53, 0.53)

Details:

Gain ref is save in data/Initiation_Complex/gain/ folder.
Total dose 60 e.
no rotation, Flip upsidedown for motioncor in RELION; no rotation, no flip in CryoSPARC.

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Browse All Files

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

CryoTRANS: predicting high-resolution maps of rare conformations from self-supervised trajectories in cryo-EM.

Fan X, Zhang Q, Zhang H, Zhu J, Ju L, Shi Z, Hu M, Bao C. (2024)

Self-assembled superstructure alleviates air-water interface effect in cryo-EM.

Zheng L, Xu J, Wang W, Gao X, Zhao C, Guo W, Sun L, Cheng H, Meng F, Chen B, Sun W, Jia X, Zhou X, Wu K, Liu Z, Ding F, Liu N, Wang HW, Peng H. (2024)

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