EMPIAR-12049 - Cryo-Electron Tomography Data and Comprehensive Annotation of C. elegans Centrosomes in Interphase and Mitosis [multiple data sets in MRC and TIFF formats]

EMPIAR-12049

Cryo-Electron Tomography Data and Comprehensive Annotation of C. elegans Centrosomes in Interphase and Mitosis [multiple data sets in MRC and TIFF formats]

Publication:

Molecular architectures of centrosomes in C. elegans embryos visualized by cryo-electron tomography

Tollervey F., Rios M.U., Zagoriy I., Woodruff J.B., Mahamid J.

(---)

DOI: 10.1016/j.devcel.2024.12.002

Related EMDB entry:

EMD-19780 EMD-19781 EMD-19778 EMD-19779

Deposited:

2024-01-23

Released:

2025-06-30

Last modified:

2025-06-30

Imageset size:

111.35 GB

Imageset DOI:

10.6019/EMPIAR-12049

Experimental metadata:

Download xml json

Contains:

reconstructed volumes
micrographs - multiframe

Image Sets

1. Raw reconstructed tomograms of C. elegans centrosomes

Category:

reconstructed volumes

Image format:

MRC

No. of images or tilt series:

Image size:

(980, 928)

Pixel type:

UNSIGNED 32 BIT INTEGER

Pixel spacing:

(13.4808, 13.4808)

Details:

Tomograms aligned in etomo and reconstructed in warp using a back projection at 4x binning. A combination of patch tracking and fiducials were used depending on the tomogram

Files:

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2. Unaligned multi-frame micrographs of C. elegans centrosomes

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

681

Image size:

(3838, 3710)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(3.37, 3.37)

Details:

Raw unaligned frame of C. elegans centrosomes, including metadata (modified and unmodified mdoc files), and alignment data (.xf, .rawtilt, and .st files). Images consist of 8 to 14 frames depending on the image set. To relate image set to centrosome number as seen in the label and tomogram directories, please refer to the readme file.

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

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Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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