EMPIAR-12025 - Tomograms and the raw 2d tilt series of ER-nuclear envelope junctions of HeLa cells in interphase [multiple data sets in TIFF and MRC formats]

EMPIAR-12025

Tomograms and the raw 2d tilt series of ER-nuclear envelope junctions of HeLa cells in interphase [multiple data sets in TIFF and MRC formats]

Publication:

The endoplasmic reticulum connects to the nucleus by constricted junctions that mature after open mitosis in mammalian cells

Bragulat-Teixidor H, Ishihara K, Szücs GM, Otsuka S

bioRxiv (2023)

DOI: 10.1101/2023.01.31.526419

Related EMDB entry:

EMD-50068

Deposited:

2024-02-14

Released:

2024-07-04

Last modified:

2024-07-04

Imageset size:

434.03 GB

Imageset DOI:

10.6019/EMPIAR-12025

Experimental metadata:

Download xml json

Contains:

tilt series
reconstructed volumes

Image Sets

1. Tilt series of ER-nuclear envelope junctions of HeLa cells in interphase

Category:

tilt series

Image format:

TIFF

No. of images or tilt series:

110

Image size:

(4096, 4096)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(4.51, 4.51)

Details:

HeLa cells were instantly frozen using a high-pressure freezing machine (High Pressure Freezer Leica EM ICE equipped with the Coral Life workflow; Leica Microsystems) and freeze-substituted into Agar 100 Epoxy resin (Agar Scientific). As fiducial markers, 15 nm of gold-conjugated Protein A (Cytodiagnostics, AC-15-05-10) was absorbed on both sides of 250 nm sections. Sections were post-stained with 2% UA and lead citrate. Dual axis tilt series were acquired with a Tecnai G2 20 transmission electron microscope operated at 200 kV and equipped with an Eagle 4k HS CCD camera (FEI) by using the Serial EM software. Images were recorded over a -55° to 55° tilt range on average with an angular increment 1°.

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2. Reconstructed tomograms of ER-nuclear envelope junctions of HeLa cells in interphase

Category:

reconstructed volumes

Image format:

MRC

No. of images or tilt series:

110

Image size:

(4096, 4096)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(4.51, 4.51)

Details:

---

Files:

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Citations

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The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

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