EMPIAR-11943 - Cryo-ET of Dictyostelium discoideum in 0.4 M sorbitol (hyperosmotic stress) [59 tilt series in MRC format]

EMPIAR-11943

Cryo-ET of Dictyostelium discoideum in 0.4 M sorbitol (hyperosmotic stress) [59 tilt series in MRC format]

Publication:

Nuclear pores as conduits for fluid flow during osmotic stress

Hoffmann PC, Kim H, Obarska-Kosinska A, Kreysing JP, Andino-Frydman E, Cruz-Leon S, Cernikova L, Kosinski J, Turonova B, Hummer G, Beck M

(---)

Related EMDB entry:

EMD-19155

Deposited:

2024-01-09

Released:

2025-02-24

Last modified:

2025-02-24

Imageset size:

332.09 GB

Imageset DOI:

10.6019/EMPIAR-11943

Experimental metadata:

Download xml json

Contains:

tilt series

Image Sets

1. Raw tilt series of D. dicoideum cells (hyperosmotic stress condition) - frame aligned

Category:

tilt series

Image format:

MRC

No. of images or tilt series:

Image size:

(None, None)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(None, None)

Details:

Tilt series were frame-aligned in SerialEM during acquisition, aligned mrc and mdoc files are provided, TS overview files contain information about acquisition parameters and excluded tilt series images from raw stacks, alignment files (.xf) and tilt files (.tlt) were generated from IMOD (note that some tilt images may be excluded, for more information see TiltSeries_Info_hypertonic.xlsx file), CTF estimation from gctf are included as gctf.star files.

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

See all citations

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