EMPIAR-11906 - UFM1 ribosome E3 ligase complex bound to the 60S ribosomal subunit [multiple data sets in EER and MRC formats]

EMPIAR-11906

UFM1 ribosome E3 ligase complex bound to the 60S ribosomal subunit [multiple data sets in EER and MRC formats]

Publication:

The UFM1 E3 ligase recognizes and releases 60S ribosomes from ER translocons

Makhlouf L, Peter JJ, Magnussen HM, Thakur R, Millrine D, Minshull TC, Harrison G, Varghese J, Lamoliatte F, Foglizzo M, Macartney T, Calabrese AN, Zeqiraj E, Kulathu Y

Nature 627 (2024) 437-444

PMID: 38383789

DOI: 10.1038/s41586-024-07093-w

Related EMDB entry:

EMD-18381 EMD-18382

Deposited:

2024-01-26

Released:

2024-07-12

Last modified:

2024-07-12

Imageset size:

24.83 TB

Imageset DOI:

10.6019/EMPIAR-11906

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
micrographs - single frame

Image Sets

1. Unaligned multiframe micrographs in EER format of the UFM1 ribosome E3 ligase complex bound to the 60S ribosome subunit

Category:

micrographs - multiframe

Image format:

EER

No. of images or tilt series:

59394

Image size:

(None, None)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(0.74, 0.74)

Details:

Dose per physical pixel/s of 6.85 used resulting in a total dose of 33.4 e/Å2. Defocus range of -0.2 to -2. For motion correction, 819 internal frames were grouped by 20 resulting in 42 EER fractions with a dose per frame of 0.8 e/A2. Gain reference file called "20221115_154105_EER_GainReference.gain".

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2. Aligned micrographs of the UFM1 ribosome E3 ligase complex bound to the 60S ribosome subunit

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

59394

Image size:

(None, None)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(None, None)

Details:

Raw movie frames are aligned with RELION's own implementation of the MotionCor2 algorithm using an EER fractionation of 20 and dose per frame of 0.8 e/A2. 5, 5 patches were used.

Files:

This entry cannot be downloaded from PDBj.

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