EMPIAR-11766 - Single-particle cryo electron microscopy of Integrin aEb7 bound to LF61 fab [multiple data sets in TIFF, MRC and MRCS formats]

EMPIAR-11766

Single-particle cryo electron microscopy of Integrin aEb7 bound to LF61 fab [multiple data sets in TIFF, MRC and MRCS formats]

Publication:

Evolutionary origin and structural ligand mimicry by the inserted domain of alpha-integrin proteins

Hollis JA, Malik HS, Campbell MG, Chan MC

(---)

Related EMDB entry:

EMD-42608

Deposited:

2023-11-02

Released:

2025-07-23

Last modified:

2025-07-23

Imageset size:

4.22 TB

Imageset DOI:

10.6019/EMPIAR-11766

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
micrographs - single frame
picked particles - single frame - processed

Image Sets

1. Unaligned multi-frame movies of clasped integrin aEb7 bound to fab LF61 in the compact state

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

4074

Image size:

(5760, 4092)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(0.561, 0.561)

Details:

αEβ7 integrin in the compact state bound to LF61 fab: 4074 unaligned uncorrected movies were recorded automatically using SerialEM on a K3 camera in super-resolution mode on a Glacios microscope operated at 200 kV (Cs=2.7). The movies contain 100 frames and the super resolution pixel size is 0.561 Å/px. The total dose per movie is ~50 e/Å^2. The gain reference is also included in .dm4 format.

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2. Dose-weighted aligned micrographs of clasped integrin aEb7 bound to fab LF61 in the compact state

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

4074

Image size:

(5760, 4092)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.122, 1.122)

Details:

αEβ7 integrin in the compact state bound to LF61 fab: 1363 unaligned uncorrected movies were recorded automatically using SerialEM on a K3 camera in super-resolution mode on a Glacios microscope operated at 200 kV (Cs=2.7). The total dose per micrograph is ~50 e/Å^2. Images were dose-weighted and motion-corrected in MotionCor2 via the RELION GUI. Images were binned by 2 using Fourier cropping and the binned pixel size is 1.122.

Files:

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3. Dose-weighted aligned particle stacks of clasped integrin aEb7 bound to fab LF61 in the compact state with a closed I domain

Category:

picked particles - single frame - processed

Image format:

MRCS

No. of images or tilt series:

402099

Image size:

(328, 328)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.122, 1.122)

Details:

Stacks of 402099 particles selected after sequential 2D and 3D classification contributing to final reconstruction of αEβ7 integrin with a closed I domain bound to LF61 fab. Unaligned uncorrected movies were recorded automatically using SerialEM on a K3 camera in super-resolution mode on a Glacios microscope operated at 200 kV (Cs=2.7). The total dose per micrograph is ~50 e/Å^2. Images were dose-weighted and motion-corrected in MotionCor2 via the RELION GUI. Images were binned by 2 using Fourier cropping and the binned pixel size is 1.122.

Files:

This entry cannot be downloaded from PDBj.

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We recommend using rsync, aspera, globus, etc.

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4. Dose-weighted aligned particle stacks of clasped integrin aEb7 bound to fab LF61 in the compact state with an open I domain

Category:

picked particles - single frame - processed

Image format:

MRCS

No. of images or tilt series:

379901

Image size:

(328, 328)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.122, 1.122)

Details:

Stacks of 379901 particles selected after sequential 2D and 3D classification contributing to final reconstruction of αEβ7 integrin with an open I domain bound to LF61 fab. Unaligned uncorrected movies were recorded automatically using SerialEM on a K3 camera in super-resolution mode on a Glacios microscope operated at 200 kV (Cs=2.7). The total dose per micrograph is ~50 e/Å^2. Images were dose-weighted and motion-corrected in MotionCor2 via the RELION GUI. Images were binned by 2 using Fourier cropping and the binned pixel size is 1.122.

Files:

This entry cannot be downloaded from PDBj.

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We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

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