EMPIAR-11654
Single particle cryo-EM dataset of mitochondrial respiratory complex I from Drosophila melanogaster [3082 multi-frame micrographs composed of 40 frames each in MRC format]
Publication:

Cryo-EM structures of mitochondrial respiratory complex I from Drosophila melanogaster

Agip AA, Chung I, Sanchez-Martinez A, Whitworth AJ, Hirst J

eLife 12 (2023)

PMID: 36622099

Related EMDB entry:
Deposited:
2023-08-02
Released:
2023-10-03
Last modified:
2023-10-03
Imageset size:
620.99 GB
Imageset DOI:
Experimental metadata:
Download xml json

Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

Movies (FoilHole*.mrc) in this entry were compressed to TIFF by EMPIAR Japan to save space and bandwidth.
The compression is lossless in that all pixel intensities remain exactly the same after decompression.
However, some metadata in the MRC header were lost.
The original files before compression are available from EMPIAR EBI.

Compression was carried out by separating (“un-normalizing”) the gain.
The details of the method are described in
https://relion.readthedocs.io/en/release-3.1/Reference/MovieCompression.html#gain-normalised-movies.

Please apply the gain reference (gain-reference.mrc) during processing.

Original size:   6,856,963,048 kilobytes
Compressed size:   651,152,769 kilobytes
Contains:
  • micrographs - multiframe
1. Unaligned, gain-corrected micrographs of mitochondrial complex I from Drosophila melanogaster
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
3082
Image size:
(3838, 3710)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.048, 1.048)
Details:
Micrographs are the average of 40 unaligned, gain-corrected frames. . Data were collected over 10 seconds with a dose rate of 4.18 e / A-2 / s with a total dose of 41.8 e/A-2.
Files:
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